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PMID: 287034 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Germ line integration of Moloney leukemia virus: identification of the chromosomal integration site.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 76 ·No. 4 ·1979-04-00 ·Pages 1938-42

Breindl M, Doehmer J, Willecke K, Dausman J, Jaenisch R

Abstract

The chromosomal integration site of the structural gene of Moloney murine leukemia virus (M-MuLV) in the genome of BALB/Mo mice was mapped genetically. These mice transmit the exogenous M-MuLV as an endogenous virus at a single Mendelian locus. Two independent experimental approaches were used: (i) Non-virus-producing fibroblasts prepared from homozygous BALB/Mo embryos were fused to Chinese hamster Wg3-h-o cells. In an analysis of 30 independent mouse-Chinese hamster cell hybrid clones, the segregation of the viral genome measured by molecular hybridization and enzymes assigned to 16 different mouse chromosomes were compared. We found a highly concordant segregation of M-MuLV sequences and the mouse enzyme triosephosphate isomerase (TPI, EC 5.3.1.1), whose gene has been assigned to chromosome 6. A further karyotype analysis of 9 clones, in which the chromosomes were identified cytochemically, supported this result. (ii) The segregation of the viral genome was studied in backcrosses of BALB/Mo with ABP/J mice. In the backcross ABP/Jx(ABP/JxBALB/Mo) a linkage of the M-MuLV genome to the morphological marker wa-1 on mouse chromosome 6 was found. This confirmed the conclusion that the M-MuLV genome is integrated in mouse chromosome 6. These experiments define the genetic locus Mov-1, denoting the genetically transmitted structural gene of M-MuLV in BALB/Mo mice.

MeSH Terms
Animals Chromosomes/physiology Clone Cells Cricetinae Crosses, Genetic Fibroblasts Genes Genotype Heterozygote Hybrid Cells Mice Mice, Inbred BALB C Moloney murine leukemia virus/genetics Recombination, Genetic
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Breindl M
Doehmer J
Willecke K
Dausman J
Jaenisch R
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36 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.76.4.1938
Published
1979-04-00
Pages
1938-42
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC383508
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.76.4.1938 PMC Full Text View journal details
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