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PMID: 2874482 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Amplification of the gene for histidyl-tRNA synthetase in histidinol-resistant Chinese hamster ovary cells.

Molecular and cellular biology ·Vol. 5 ·No. 9 ·1985-09-00 ·Pages 2381-8

Tsui FW, Andrulis IL, Murialdo H, Siminovitch L

Abstract

Histidinol-resistant (HisOHR) mutants with up to a 30-fold increase in histidyl-tRNA synthetase activity have been isolated by stepwise adaptation of wild-type Chinese hamster ovary (CHO) cells to increasing amounts of histidinol in the medium. Immunoprecipitation of [35S]methionine-labeled cell lysates with antibodies to histidyl-tRNA synthetase showed increased synthesis of the enzyme in histidinol-resistant cells. The histidinol-resistant cell lines had an increase in translatable polyadenylated mRNA for histidyl-tRNA synthetase. A cDNA for CHO histidyl-tRNA synthetase has been cloned, using these histidyl-tRNA synthetase-overproducing mutants as the source of mRNA. Southern blot analysis of wild-type and histidinol-resistant cells with this cDNA showed that the histidyl-tRNA synthetase DNA bands were amplified in the resistant cells. These HisOHR cells owed their resistance to histidinol to amplification of the gene for histidyl-tRNA synthetase.

MeSH Terms
Amino Acyl-tRNA Synthetases/genetics Animals Cell Line Cricetinae Cricetulus DNA Drug Resistance Female Fibroblasts/drug effects Gene Amplification Histidine-tRNA Ligase/genetics Histidinol/pharmacology Molecular Weight Poly A/biosynthesis RNA, Messenger/biosynthesis
Chemicals
RNA, Messenger Poly A Histidinol DNA Amino Acyl-tRNA Synthetases Histidine-tRNA Ligase
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Tsui F W
Andrulis I L
Murialdo H
Siminovitch L
References (23)
23 references, click to expand
  1. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
  2. Protein measurement with the Folin phenol reagent.
    J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
  3. A mammalian cell mutant with a temperature-sensitive leucyl-transfer RNA synthetase.
    Proc Natl Acad Sci U S A. 1973 Nov;70(11):3094-8 PMID: 4361675
  4. A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels.
    Eur J Biochem. 1974 Jul 1;46(1):83-8 PMID: 4850204
  5. Cell membrane antigen isolation with the staphylococcal protein A-antibody adsorbent.
    J Immunol. 1976 Nov;117(5 Pt 1):1482-90 PMID: 187693
  6. Selection by [3H] amino acids of CHO-cell mutants with altered leucyl- and asparagyl-transfer RNA synthetases.
    Somatic Cell Genet. 1975 Apr;1(2):187-208 PMID: 1235906
  7. Biochemical characterization of a mutant asparaginyl-tRNA synthetase from Chinese hamster ovary cells.
    J Biol Chem. 1978 Jan 10;253(1):58-62 PMID: 618867
  8. Six complementation classes of conditionally lethal protein synthesis mutants of CHO cells selected by 3H-amino acid.
    Somatic Cell Genet. 1978 Jan;4(1):27-44 PMID: 628883
  9. Evidence for structural gene alterations affecting aminoacyl-tRNA synthetases in CHO cell mutants and revertants.
    Somatic Cell Genet. 1978 Jul;4(4):423-35 PMID: 684557
  10. Mutations in the structural genes of CHO cell histidyl-, valyl-, and leucyl-tRNA synthetases.
    Somatic Cell Genet. 1978 May;4(3):299-312 PMID: 694722
  11. N-(Phosphonacetyl)-L-aspartate-resistant hamster cells overaccumulate a single mRNA coding for the multifunctional protein that catalyzes the first steps of UMP synthesis.
    J Biol Chem. 1979 Feb 10;254(3):974-80 PMID: 762107
  12. Construction and characterization of pro alpha 1 collagen complementary deoxyribonucleic acid clones.
    Biochemistry. 1979 Jul 10;18(14):3146-52 PMID: 380642
  13. Gene amplification causes overproduction of the first three enzymes of UMP synthesis in N-(phosphonacetyl)-L-aspartate-resistant hamster cells.
    J Biol Chem. 1979 Sep 10;254(17):8679-89 PMID: 381311
  14. Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease.
    Biochemistry. 1979 Nov 27;18(24):5294-9 PMID: 518835
  15. Role of aminoacylation of histidyl-tRNA in the regulation of protein degradation in Chinese hamster ovary cells.
    J Biol Chem. 1980 Jul 10;255(13):6322-9 PMID: 7391021
  16. Myositis autoantibody inhibits histidyl-tRNA synthetase: a model for autoimmunity.
    Nature. 1983 Jul 14-20;304(5922):177-9 PMID: 6866113
  17. Gene amplification in cultured animal cells.
    Cell. 1984 Jul;37(3):705-13 PMID: 6378386
  18. Chinese hamster ovary cells resistant to borrelidin overproduce threonyl-tRNA synthetase.
    J Biol Chem. 1984 Jul 25;259(14):9202-6 PMID: 6746646
  19. Threonyl-tRNA synthetase from Chinese hamster ovary cells is phosphorylated on serine.
    J Biol Chem. 1984 Sep 25;259(18):11160-1 PMID: 6432793
  20. Gene amplification.
    Annu Rev Biochem. 1984;53:447-91 PMID: 6383198
  21. Isolation of Chinese hamster ovary cells that overproduce asparaginyl-tRNA synthetase.
    Mol Cell Biol. 1984 Sep;4(9):1939-41 PMID: 6493235
  22. Expression of several amplified genes in an adenylate-deaminase overproducing variant of Chinese hamster fibroblasts.
    EMBO J. 1984 Dec 20;3(13):3123-7 PMID: 6526013
  23. Two types of ribosome in mouse-hamster hybrid cells.
    Nat New Biol. 1971 Mar 10;230(10):52-4 PMID: 5279808
Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.5.9.2381-2388.1985
Published
1985-09-00
Pages
2381-8
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC366965
Subset
IM
External Links
PubMed source DOI: 10.1128/mcb.5.9.2381-2388.1985 PMC Full Text View journal details
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