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PMID: 2880806 Published · ppublish English Journal Article

Stimulation of human polymorphonuclear leukocyte oxidative metabolism by type 1 pili from Escherichia coli.

Infection and immunity ·Vol. 55 ·No. 3 ·1987-03-00 ·Pages 534-40

Goetz MB, Silverblatt FJ

Abstract

We compared the degree to which Escherichia coli phase variants which do (T1P+ E. coli) or do not (T1P- E. coli) express type 1 pili (T1P) stimulate human polymorphonuclear leukocyte (PMN) oxidative activity. Unopsonized T1P+ E. coli stimulated the release of 0.20 to 0.24 nmol of H2O2 per 10(6) PMN per min and the consumption of 1.4 to 4.0 nmol of O2 per 10(6) PMN per min; no measurable PMN oxidative activity was stimulated by unopsonized T1P- E. coli. In the presence of serum opsonins, T1P+ E. coli stimulated the release of 1.12 to 1.16 nmol of H2O2 per 10(6) PMN per min and the consumption of 5.0 to 6.0 nmol of O2 per 10(6) PMN per min, whereas T1P- E. coli stimulated the release of 0.42 to 0.43 nmol of H2O2 per 10(6) PMN per min and the consumption of 0.6 to 2.0 nmol of O2 per 10(6) PMN per min. Although unaggregated T1P did not stimulate PMN, latex beads coated with T1P (T1P-latex) stimulated alpha-methylmannoside-inhibitable, opsonin-independent PMN oxidative activity. The activity stimulated by either T1P+ E. coli or T1P-latex was susceptible to inhibition by cytochalasin B. Latex particles coated with bovine serum albumin or mannose-resistant pili did not stimulate PMN. These data indicate that T1P+ E. coli stimulate PMN oxidative metabolism more effectively than do T1P- E. coli and that a similar PMN oxidative response follows cellular stimulation by either unopsonized T1P+ or opsonized T1P- E. coli. Furthermore, T1P-latex faithfully mimics the ability of T1P+ E. coli to stimulate PMN oxidative metabolism. Such particles may be useful in further analyses of cellular responses to T1P+ E. coli.

MeSH Terms
Concanavalin A/pharmacology Cytochalasin B/pharmacology Escherichia coli/physiology Fimbriae, Bacterial/physiology Humans Hydrogen Peroxide/biosynthesis Latex/pharmacology Neutrophils/drug effects,metabolism Opsonin Proteins/immunology Oxygen Consumption Polymyxin B/pharmacology
Chemicals
Latex Opsonin Proteins Concanavalin A Cytochalasin B Hydrogen Peroxide Polymyxin B
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Goetz M B
Silverblatt F J
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.55.3.534-540.1987
Published
1987-03-00
Pages
534-40
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC260369
Subset
IM
External Links
PubMed source DOI: 10.1128/iai.55.3.534-540.1987 PMC Full Text View journal details
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