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PMID: 288081 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Isolation and characterization of somatostatin from pigeon pancreas.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 76 ·No. 6 ·1979-06-00 ·Pages 2974-8

Spiess J, Rivier JE, Rodkey JA, Bennett CD, Vale W

Abstract

Most of the somatostatin-like activity from pigeon pancreas was found to correspond to small species with an apparent molecular weight of 1500--2500. This species was isolated under conditions minimizing intermolecular interactions and protease activities. The isolated product was characterized by two somatostatin radioimmunoassays, a bioassay, endgroup determination, and amino acid analysis. The structure of the isolated compound was determined to be H-Ala-Gly-cyclo-(Cys-Lys-Asn-Phe-Phe-Trp-Lys-Thr-Phe-Thr-Ser-Cys)-OH. Additionally, small amounts of des-Ala1-somatostatin, a possible degradation product of pancreatic somatostatin, and a large somatostatin-like species with an apparent molecular weight of 11,000--12,500 were detected. It is concluded that the main somatostatin-like polypeptide isolated from pigeon pancreas is identical to the mammalian hypothalamic tetradecapeptide somatostatin.

MeSH Terms
Amino Acid Sequence Amino Acids/analysis Animals Biological Assay Columbidae Growth Hormone/metabolism Pancreas/analysis Pituitary Gland, Anterior/drug effects,metabolism Rats Somatostatin/isolation & purification,pharmacology
Chemicals
Amino Acids Somatostatin Growth Hormone
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Spiess J
Rivier J E
Rodkey J A
Bennett C D
Vale W
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20 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.76.6.2974
Published
1979-06-00
Pages
2974-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC383733
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.76.6.2974 PMC Full Text View journal details
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