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PMID: 2883656 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Molecular cloning and expression of T11 cDNAs reveal a receptor-like structure on human T lymphocytes.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 84 ·No. 9 ·1987-05-00 ·Pages 2941-5

Sayre PH, Chang HC, Hussey RE, Brown NR, Richardson NE, Spagnoli G, Clayton LK, Reinherz EL

Abstract

The T11 (CD2) sheep-erythrocyte-binding protein is a T-cell surface molecule involved in activation of T lymphocytes and thymocytes, including those lacking the T3-Ti antigen-receptor complex. The primary structure of T11 was deduced from protein microsequencing and cDNA cloning. The mature human protein appears to be divided into three domains: a hydrophilic 185 amino acid external domain bearing only limited homology to the T-cell surface protein T4 and the immunoglobulin kappa light chain variable region, a 25 amino acid hydrophobic transmembrane segment, and a 126 amino acid cytoplasmic domain rich in prolines and basic residues. Transfection of cDNAs encoding either the 1.7- or the 1.3-kilobase T11 mRNA into COS-1 cells resulted in expression of surface T11 epitopes as well as sheep-erythrocyte-binding capacity. The predicted structure is consistent with the possibility that T11 functions in signal transduction.

MeSH Terms
Amino Acid Sequence Animals Antigens, Surface/genetics Base Sequence CD2 Antigens Carrier Proteins/genetics Cell Line Cloning, Molecular DNA/metabolism Genes Humans RNA, Messenger/genetics Receptors, Antigen, T-Cell/genetics Receptors, Immunologic/genetics T-Lymphocytes/immunology Transcription, Genetic
Chemicals
Antigens, Surface CD2 Antigens Carrier Proteins RNA, Messenger Receptors, Antigen, T-Cell Receptors, Immunologic DNA
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Sayre P H
Chang H C
Hussey R E
Brown N R
Richardson N E
Spagnoli G
Clayton L K
Reinherz E L
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.84.9.2941
Published
1987-05-00
Pages
2941-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC304776
Subset
IM
Grants
NIAID NIH HHS · AI19807 · United States
NIAID NIH HHS · AI23390 · United States
NIAID NIH HHS · R01 AI21226 · United States
Databases
GENBANK
M16336
External Links
PubMed source DOI: 10.1073/pnas.84.9.2941 PMC Full Text View journal details
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