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PMID: 2885805 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Human mRNA polyadenylate binding protein: evolutionary conservation of a nucleic acid binding motif.

Nucleic acids research ·Vol. 15 ·No. 12 ·1987-06-25 ·Pages 4771-87

Grange T, de Sa CM, Oddos J, Pictet R

Abstract

We have isolated a full length cDNA (cDNA) coding for the human poly(A) binding protein. The cDNA derived 73 kd basic translation product has the same Mr, isoelectric point and peptidic map as the poly(A) binding protein. DNA sequence analysis reveals a 70,244 dalton protein. The N terminal part, highly homologous to the yeast poly(A) binding protein, is sufficient for poly(A) binding activity. This domain consists of a four-fold repeated unit of approximately 80 amino acids present in other nucleic acid binding proteins. In the C terminal part there is, as in the yeast protein, a sequence of approximately 150 amino acids, rich in proline, alanine and glutamine which together account for 48% of the residues. A 2,9 kb mRNA corresponding to this cDNA has been detected in several vertebrate cell types and in Drosophila melanogaster at every developmental stage including oogenesis.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Biological Evolution Carrier Proteins/genetics,metabolism DNA/genetics DNA, Recombinant Drosophila melanogaster/genetics Ducks/genetics Humans Poly A/metabolism Poly(A)-Binding Proteins RNA, Messenger/metabolism Rats/genetics Saccharomyces cerevisiae/genetics Sequence Homology, Nucleic Acid Xenopus laevis/genetics
Chemicals
Carrier Proteins DNA, Recombinant Poly(A)-Binding Proteins RNA, Messenger Poly A DNA
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Grange T
de Sa C M
Oddos J
Pictet R
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36 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/15.12.4771
Published
1987-06-25
Pages
4771-87
Language
English
Region
England
NLM ID
0411011
PMCID
PMC305917
Subset
IM
Databases
GENBANK
Y00345
External Links
PubMed source DOI: 10.1093/nar/15.12.4771 PMC Full Text View journal details
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