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PMID: 2885835 Published · ppublish English Comparative Study Journal Article

Characterization of the promoter region of the human c-erbB-2 protooncogene.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 84 ·No. 13 ·1987-07-00 ·Pages 4374-8

Ishii S, Imamoto F, Yamanashi Y, Toyoshima K, Yamamoto T

Abstract

Three overlapping genomic clones that contain the 5'-terminal portion of the human c-erbB-2 gene (ERBB2) were isolated. The promoter region was identified by nuclease S1 mapping with c-erbB-2 mRNA. Seven transcriptional start sites were identified. DNA sequence analysis showed that the promoter region contains a "TATA box" and a "CAAT box" about 30 and 80 base pairs (bp), respectively, upstream of the most downstream RNA initiation site. Two putative binding sites for transcription factor Sp1 were identified about 50 and 110 bp upstream of the CAAT box, and six GGA repeats were found between the CAAT box and the TATA box. This region had strong promoter activity when placed upstream of the bacterial chloramphenicol acetyltransferase gene and transfected into monkey CV-1 cells. These data indicate that the promoter of the human c-erbB-2 protooncogene is different from that of the protooncogene c-erbB-1 (epidermal growth factor receptor gene), which does not contain either a TATA box or a CAAT box. Comparison of the promoter sequences and activities of the two protooncogenes should be helpful in analysis of the regulatory mechanism of expression of their gene products, which are growth-factor receptors.

MeSH Terms
Base Sequence DNA/genetics DNA, Recombinant/analysis ErbB Receptors/genetics Fetus/analysis Gene Expression Regulation Humans Promoter Regions, Genetic Proto-Oncogene Proteins/genetics Proto-Oncogenes RNA, Messenger/analysis Receptor, ErbB-2 Recombinant Fusion Proteins/biosynthesis Transcription, Genetic
Chemicals
DNA, Recombinant Proto-Oncogene Proteins RNA, Messenger Recombinant Fusion Proteins DNA ErbB Receptors Receptor, ErbB-2
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Ishii S
Imamoto F
Yamanashi Y
Toyoshima K
Yamamoto T
References (36)
36 references, click to expand
  1. Organization and expression of eucaryotic split genes coding for proteins.
    Annu Rev Biochem. 1981;50:349-83 PMID: 6791577
  2. A system for shotgun DNA sequencing.
    Nucleic Acids Res. 1981 Jan 24;9(2):309-21 PMID: 6259625
  3. Recombinant genomes which express chloramphenicol acetyltransferase in mammalian cells.
    Mol Cell Biol. 1982 Sep;2(9):1044-51 PMID: 6960240
  4. The Rous sarcoma virus long terminal repeat is a strong promoter when introduced into a variety of eukaryotic cells by DNA-mediated transfection.
    Proc Natl Acad Sci U S A. 1982 Nov;79(22):6777-81 PMID: 6294651
  5. A new pair of M13 vectors for selecting either DNA strand of double-digest restriction fragments.
    Gene. 1982 Oct;19(3):269-76 PMID: 6295880
  6. Deletion mapping of DNA regions required for SV40 early region promoter function in vivo.
    J Mol Appl Genet. 1982;1(5):457-81 PMID: 6296253
  7. Isolation of transcription factors that discriminate between different promoters recognized by RNA polymerase II.
    Cell. 1983 Mar;32(3):669-80 PMID: 6187469
  8. High efficiency DNA-mediated transformation of primate cells.
    Science. 1983 Aug 5;221(4610):551-3 PMID: 6306768
  9. A synthetic heat-shock promoter element confers heat-inducibility on the herpes simplex virus thymidine kinase gene.
    EMBO J. 1982;1(11):1473-7 PMID: 6327271
  10. A sequence conserved in both the chicken and mouse alpha 2(I) collagen promoter contains sites sensitive to S1 nuclease.
    J Biol Chem. 1984 May 25;259(10):6636-40 PMID: 6327689
  11. Human epidermal growth factor receptor cDNA is homologous to a variety of RNAs overproduced in A431 carcinoma cells.
    Nature. 1984 Jun 28-Jul 4;309(5971):806-10 PMID: 6330563
  12. Multiple specific contacts between a mammalian transcription factor and its cognate promoters.
    Nature. 1984 Nov 29-Dec 5;312(5993):409-13 PMID: 6095100
  13. Characterization of epidermal growth factor receptor gene expression in malignant and normal human cell lines.
    Proc Natl Acad Sci U S A. 1984 Dec;81(23):7308-12 PMID: 6095284
  14. Stimulation of in vitro transcription by the upstream element of the adenovirus-2 major late promoter involves a specific factor.
    Nucleic Acids Res. 1984 Dec 11;12(23):8779-99 PMID: 6096806
  15. Characterization and sequence of the promoter region of the human epidermal growth factor receptor gene.
    Proc Natl Acad Sci U S A. 1985 Aug;82(15):4920-4 PMID: 2991899
  16. Sequences required for in vitro transcriptional activation of a Drosophila hsp 70 gene.
    Cell. 1985 Sep;42(2):527-37 PMID: 4028160
  17. Two distinct transcription factors bind to the HSV thymidine kinase promoter in vitro.
    Cell. 1985 Sep;42(2):559-72 PMID: 2992804
  18. A v-erbB-related protooncogene, c-erbB-2, is distinct from the c-erbB-1/epidermal growth factor-receptor gene and is amplified in a human salivary gland adenocarcinoma.
    Proc Natl Acad Sci U S A. 1985 Oct;82(19):6497-501 PMID: 2995967
  19. Cell-specific expression of the rat insulin gene: evidence for role of two distinct 5' flanking elements.
    Science. 1985 Nov 22;230(4728):912-6 PMID: 3904002
  20. High incidence of amplification of the epidermal growth factor receptor gene in human squamous carcinoma cell lines.
    Cancer Res. 1986 Jan;46(1):414-6 PMID: 2998610
  21. Similarity of protein encoded by the human c-erb-B-2 gene to epidermal growth factor receptor.
    Nature. 1986 Jan 16-22;319(6050):230-4 PMID: 3003577
  22. Human HSP70 promoter contains at least two distinct regulatory domains.
    Proc Natl Acad Sci U S A. 1986 Feb;83(3):629-33 PMID: 3456160
  23. Homologous recognition of a promoter domain common to the MSV LTR and the HSV tk gene.
    Cell. 1986 Feb 28;44(4):565-76 PMID: 3004739
  24. Amplification of c-erbB-2 oncogene in human adenocarcinomas in vivo.
    Lancet. 1986 Apr 5;1(8484):765-7 PMID: 2870269
  25. Multiple independent activations of the neu oncogene by a point mutation altering the transmembrane domain of p185.
    Cell. 1986 Jun 6;45(5):649-57 PMID: 2871941
  26. Structure and function of the S1 nuclease-sensitive site in the adenovirus late promoter.
    Cell. 1986 Jun 6;45(5):743-51 PMID: 3011277
  27. The product of the human c-erbB-2 gene: a 185-kilodalton glycoprotein with tyrosine kinase activity.
    Science. 1986 Jun 27;232(4758):1644-6 PMID: 3012781
  28. Purification and biochemical characterization of the promoter-specific transcription factor, Sp1.
    Science. 1986 Oct 3;234(4772):47-52 PMID: 3529394
  29. Localization of a novel v-erbB-related gene, c-erbB-2, on human chromosome 17 and its amplification in a gastric cancer cell line.
    Mol Cell Biol. 1986 Mar;6(3):955-8 PMID: 2430175
  30. Detection of specific sequences among DNA fragments separated by gel electrophoresis.
    J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
  31. Sizing and mapping of early adenovirus mRNAs by gel electrophoresis of S1 endonuclease-digested hybrids.
    Cell. 1977 Nov;12(3):721-32 PMID: 922889
  32. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  33. The isolation of structural genes from libraries of eucaryotic DNA.
    Cell. 1978 Oct;15(2):687-701 PMID: 719759
  34. Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease.
    Biochemistry. 1979 Nov 27;18(24):5294-9 PMID: 518835
  35. Sequencing end-labeled DNA with base-specific chemical cleavages.
    Methods Enzymol. 1980;65(1):499-560 PMID: 6246368
  36. A regulatory upstream promoter element in the Drosophila hsp 70 heat-shock gene.
    Cell. 1982 Sep;30(2):517-28 PMID: 6814763
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.84.13.4374
Published
1987-07-00
Pages
4374-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC305091
Subset
IM
Databases
GENBANK
M16892
External Links
PubMed source DOI: 10.1073/pnas.84.13.4374 PMC Full Text View journal details
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