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PMID: 2888886 Published · ppublish English Journal Article

A fast electrophoretic isoenzyme technique for the identification of invasive and non-invasive Entamoeba histolytica and "E. histolytica-like" organisms.

The Journal of protozoology ·Vol. 34 ·No. 3 ·1987-08-00 ·Pages 253-5

Moss DM, Mathews HM

Abstract

Cellulose acetate electrophoresis (CAE) was used to separate glucosephosphate isomerase, hexokinase, malic enzyme, and phosphoglucomutase extracted from invasive and non-invasive Entamoeba histolytica and "E. histolytica-like" organisms. Each of these morphologically similar organisms possessed a unique CAE isoenzyme profile that can be used as an aid in their identification. The CAE technique used to obtain these isoenzyme profiles is rapid, simple, and economical, and it requires neither specialized training nor elaborate equipment.

MeSH Terms
Animals Electrophoresis, Cellulose Acetate Entamoeba histolytica/classification,enzymology Glucose-6-Phosphate Isomerase/analysis Hexokinase/analysis Humans Isoenzymes/analysis Malate Dehydrogenase/analysis Phosphoglucomutase/analysis
Chemicals
Isoenzymes Malate Dehydrogenase Hexokinase Glucose-6-Phosphate Isomerase Phosphoglucomutase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Moss D M
U.S. Department of Health and Human Services, Centers for Disease Control, Atlanta, Georgia 30333.
Mathews H M
Article Info
Journal
The Journal of protozoology
Abbr.
J Protozool
ISSN
0022-3921
Published
1987-08-00
Pages
253-5
Language
English
Region
United States
NLM ID
2985197R
Subset
IM
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