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PMID: 2892795 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

The Mycobacterium tuberculosis 65-kilodalton antigen is a heat shock protein which corresponds to common antigen and to the Escherichia coli GroEL protein.

Infection and immunity ·Vol. 56 ·No. 2 ·1988-02-00 ·Pages 446-51

Shinnick TM, Vodkin MH, Williams JC

Abstract

Monoclonal hybridoma antibodies directed against a 65-kilodalton (kDa) mycobacterial protein could detect similarly sized antigens in many other bacterial species. In Pseudomonas aeruginosa, the cross-reacting protein corresponded to a 62-kDa antigen that has been called Common Antigen. The mycobacterial 65-kDa antigen and Common Antigen are similar in that both (i) are highly immunoreactive molecules, (ii) contain species-specific and genus-specific epitopes in addition to the broadly cross-reactive epitopes, (iii) can be isolated as homomultimers of greater than 240 kDa, and (iv) have similar amino acid compositions. In Escherichia coli, the cross-reactive protein corresponded to the GroEL protein. Both the GroEL protein and the mycobacterial 65-kDa protein are expressed as heat shock proteins.

MeSH Terms
Antigens, Bacterial/immunology Bacterial Proteins/immunology Chaperonin 60 Cross Reactions Escherichia coli/physiology Genes, Bacterial Heat-Shock Proteins/immunology Molecular Weight Mycobacterium tuberculosis/immunology
Chemicals
Antigens, Bacterial Bacterial Proteins Chaperonin 60 Heat-Shock Proteins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Shinnick T M
Division of Bacterial Diseases, Centers for Disease Control, Atlanta, Georgia 30333.
Vodkin M H
Williams J C
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.56.2.446-451.1988
Published
1988-02-00
Pages
446-51
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC259302
Subset
IM
Grants
NIAID NIH HHS · AI22217 · United States
External Links
PubMed source DOI: 10.1128/iai.56.2.446-451.1988 PMC Full Text View journal details
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