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PMID: 2893786 Published · ppublish English Journal Article

Localization and enumeration of fimbria-associated adhesins of Bacteroides loescheii.

Journal of bacteriology ·Vol. 170 ·No. 3 ·1988-03-00 ·Pages 1123-8

Weiss EI, London J, Kolenbrander PE, Hand AR, Siraganian R

Abstract

Monoclonal antibodies that specifically inhibit coaggregation between Bacteroides loescheii PK1295 and its two gram-positive partners Streptococcus sanguis 34 and Actinomyces israelii PK14 were used to enumerate and localize two distinct types of fimbria-associated adhesins on the surface of B. loescheii. Binding studies with radiolabeled monoclonal antibodies indicated that a maximum (Bmax calculated from Scatchard plots) of approximately 400 adhesin molecules specific for S. sanguis and 310 adhesin molecules specific for A. israelii reside on the surface of the cell. Immunoelectron microscopy revealed that the adhesins were not an integral part of the fimbrial subunit; rather, they were usually found on the distal portion of the structures arranged in a random fashion.

MeSH Terms
Antibodies, Monoclonal Bacterial Adhesion Bacterial Proteins/analysis,immunology Bacteroides/analysis,ultrastructure Fimbriae, Bacterial/immunology,physiology Immunohistochemistry Microscopy, Electron
Chemicals
Antibodies, Monoclonal Bacterial Proteins
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Weiss E I
Laboratory of Microbiology and Immunology, National Institute of Dental Research, Bethesda, Maryland 20892.
London J
Kolenbrander P E
Hand A R
Siraganian R
References (19)
19 references, click to expand
  1. A graphic method for the determination and presentation of binding parameters in a complex system.
    Anal Biochem. 1967 Sep;20(3):525-32 PMID: 6048188
  2. Monoclonal antibodies raised against Pap fimbriae recognize minor component(s) involved in receptor binding.
    Microb Pathog. 1987 Feb;2(2):113-21 PMID: 2907082
  3. Host-specific fimbrial adhesins of noninvasive enterotoxigenic Escherichia coli strains.
    Microbiol Rev. 1982 Jun;46(2):129-61 PMID: 6126799
  4. Proteinaceous bacterial adhesins and their receptors.
    Crit Rev Microbiol. 1983;10(3):229-60 PMID: 6138214
  5. Purification and characterization of a novel type of fimbriae from the oral anaerobe Bacteroides gingivalis.
    J Bacteriol. 1984 Dec;160(3):949-57 PMID: 6150029
  6. Adhesion to human cells by Escherichia coli lacking the major subunit of a digalactoside-specific pilus-adhesin.
    Proc Natl Acad Sci U S A. 1985 Mar;82(6):1800-4 PMID: 2858852
  7. Globoside-specific adhesins of uropathogenic Escherichia coli are encoded by similar trans-complementable gene clusters.
    J Bacteriol. 1985 Jun;162(3):1293-301 PMID: 2860097
  8. Fimbriae from the oral anaerobe Bacteroides gingivalis: physical, chemical, and immunological properties.
    J Bacteriol. 1985 Aug;163(2):730-4 PMID: 2862134
  9. Fimbrial adhesions of Escherichia coli.
    Rev Infect Dis. 1985 May-Jun;7(3):321-40 PMID: 2862689
  10. Genes determining adhesin formation in uropathogenic Escherichia coli.
    Curr Top Microbiol Immunol. 1985;118:163-78 PMID: 2865087
  11. Fimbriation and P-antigen recognition of Escherichia coli strains harbouring mutated recombinant plasmids encoding fimbrial adhesins of the uropathogenic E. coli strain KS71.
    J Gen Microbiol. 1986 Jan;132(1):71-7 PMID: 2872265
  12. Multigeneric aggregations among oral bacteria: a network of independent cell-to-cell interactions.
    J Bacteriol. 1986 Nov;168(2):851-9 PMID: 3782029
  13. The transfer of genes encoding production of mannose-resistant haemagglutinating fimbriae from uropathogenic enterobacteria.
    J Gen Microbiol. 1986 Aug;132(8):2243-7 PMID: 2878966
  14. Adherence to human colonocytes of an Escherichia coli strain isolated from severe infantile enteritis: molecular and ultrastructural studies of a fibrillar adhesin.
    Infect Immun. 1987 Feb;55(2):393-402 PMID: 2879795
  15. Isolation and characterization of the alpha-sialyl-beta-2,3-galactosyl-specific adhesin from fimbriated Escherichia coli.
    Proc Natl Acad Sci U S A. 1987 May;84(10):3462-6 PMID: 2883653
  16. Localization of the receptor-binding protein adhesin at the tip of the bacterial pilus.
    Nature. 1987 Jul 2-8;328(6125):84-7 PMID: 2885755
  17. Fimbria-associated proteins of Bacteroides loescheii PK1295 mediate intergeneric coaggregations.
    J Bacteriol. 1987 Sep;169(9):4215-22 PMID: 2887546
  18. Characterization of monoclonal antibodies to fimbria-associated adhesins of Bacteroides loescheii PK1295.
    Infect Immun. 1988 Jan;56(1):219-24 PMID: 2891617
  19. A low-viscosity epoxy resin embedding medium for electron microscopy.
    J Ultrastruct Res. 1969 Jan;26(1):31-43 PMID: 4887011
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.170.3.1123-1128.1988
Published
1988-03-00
Pages
1123-8
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC210881
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.170.3.1123-1128.1988 PMC Full Text View journal details
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