Home LiteratureArticle Details
PMID: 2900460 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Regulation of pyrC expression in Salmonella typhimurium: identification of a regulatory region.

Molecular & general genetics : MGG ·Vol. 212 ·No. 2 ·1988-05-00 ·Pages 287-94

Kelln RA, Neuhard J

Abstract

Deletion analysis of a plasmid carrying the entire pyrC gene of Salmonella typhimurium served to localize the regulatory region within a 120 base pair DNA fragment comprising the promoter-leader region and the first 10 codons of pyrC. A region of dyad symmetry is present in the leader DNA and may result in the formation of a stable hairpin in the transcript with part of the Shine-Dalgarno sequence included in the stem. Four independently-isolated regulatory mutants, overexpressing pyrC, were found to have point mutations within the symmetry region and, significantly, the mutations occurred in sequences pertaining to either side of the stem of the putative hairpin of the transcript. All four mutations would decrease the stability of the hairpin, suggesting that pyrC expression is controlled at the level of translation. Additional evidence for translational control was provided by the finding that synthesis of galactokinase mediated from a pyrC-galK transcriptional fusion is not regulated by pyrimidines. The importance of the symmetry region in the leader was further emphasized by showing that pyrC expression is strongly affected when this region is deleted, inverted, or structured as a tandem duplication.

MeSH Terms
Amidohydrolases/genetics Base Sequence DNA Restriction Enzymes Dihydroorotase/genetics Gene Expression Regulation Genes Genes, Bacterial Genes, Regulator Molecular Sequence Data Nucleic Acid Conformation Plasmids Promoter Regions, Genetic Salmonella typhimurium/enzymology,genetics
Chemicals
DNA Restriction Enzymes Amidohydrolases Dihydroorotase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Kelln R A
Department of Chemistry, University of Regina, Saskatchewan, Canada.
Neuhard J
References (32)
32 references, click to expand
  1. Pyrimidine biosynthetic enzymes of Salmonella typhimurium, repressed specifically by growth in the presence of cytidine.
    J Bacteriol. 1975 Nov;124(2):764-74 PMID: 1102530
  2. Improved plasmid vectors for the isolation of translational lac gene fusions.
    Gene. 1984 Nov;31(1-3):269-73 PMID: 6098531
  3. Structure of the Escherichia coli pyrE operon and control of pyrE expression by a UTP modulated intercistronic attentuation.
    EMBO J. 1984 Aug;3(8):1783-90 PMID: 6207018
  4. Conditional lethal mutants of the small filamentous coliphage M13. I. Isolation, complementation, cell killing, time of cistron action.
    Virology. 1966 Nov;30(3):397-410 PMID: 5921643
  5. Translational initiation in prokaryotes.
    Annu Rev Microbiol. 1981;35:365-403 PMID: 6170248
  6. Cloning and characterization of the pyrE gene and of PyrE::Mud1 (Ap lac) fusions from Salmonella typhimurium.
    Eur J Biochem. 1985 Feb 1;146(3):597-603 PMID: 3882417
  7. Control of expression of the pyr genes in Salmonella typhimurium: effects of variations in uridine and cytidine nucleotide pools.
    J Bacteriol. 1975 Mar;121(3):814-22 PMID: 163814
  8. Role of translation and attenuation in the control of pyrBI operon expression in Escherichia coli K-12.
    J Bacteriol. 1985 Sep;163(3):991-9 PMID: 3928602
  9. DNA-protein recognition: demonstration of three genetically separated operator elements that are required for repression of the Escherichia coli deoCABD promoters by the DeoR repressor.
    EMBO J. 1986 Aug;5(8):2015-21 PMID: 3019678
  10. Repression of Escherichia coli carbamoylphosphate synthase: relationships with enzyme synthesis in the arginine and pyrimidine pathways.
    J Bacteriol. 1976 Jul;127(1):291-301 PMID: 179975
  11. Cloning and characterization of the pyrF operon of Salmonella typhimurium.
    Eur J Biochem. 1987 May 4;164(3):613-9 PMID: 2436909
  12. Alterations upstream from the Shine-Dalgarno region and their effect on bacterial gene expression.
    Gene. 1985;36(3):211-23 PMID: 3000873
  13. Enhanced expression of cro-beta-galactosidase fusion proteins under the control of the PR promoter of bacteriophage lambda.
    EMBO J. 1982;1(10):1217-24 PMID: 6327257
  14. New M13 vectors for cloning.
    Methods Enzymol. 1983;101:20-78 PMID: 6310323
  15. Cloning, nucleotide sequence and expression of the pyrBI operon of Salmonella typhimurium LT2.
    Eur J Biochem. 1987 Jul 1;166(1):55-61 PMID: 3036524
  16. Regulation of aspartate transcarbamoylase synthesis in Escherichia coli: analysis of deletion mutations in the promoter region of the pyrBI operon.
    Proc Natl Acad Sci U S A. 1985 Jul;82(14):4643-7 PMID: 2991885
  17. Cloning and structural characterization of the Salmonella typhimurium pyrC gene encoding dihydroorotase.
    Eur J Biochem. 1986 Jun 2;157(2):335-42 PMID: 2872051
  18. A system to study promoter and terminator signals recognized by Escherichia coli RNA polymerase.
    Gene Amplif Anal. 1981;2:383-415 PMID: 6101056
  19. Nucleotide sequence of the structural gene for dihydroorotase of Escherichia coli K12.
    Eur J Biochem. 1986 Oct 1;160(1):77-82 PMID: 2876892
  20. Mechanism of UTP-modulated attenuation at the pyrE gene of Escherichia coli: an example of operon polarity control through the coupling of translation to transcription.
    EMBO J. 1984 Dec 1;3(12):2857-61 PMID: 6098450
  21. Effect of UTP and GTP pools on attenuation at the pyrE gene of Escherichia coli.
    Mol Gen Genet. 1987 Jun;208(1-2):152-8 PMID: 3302606
  22. Studies on the structure and expression of Escherichia coli pyrC, pyrD, and pyrF using the cloned genes.
    Eur J Biochem. 1984 Apr 16;140(2):343-52 PMID: 6370696
  23. Rapid mutational analysis of regulatory loci in Escherichia coli K-12 using bacteriophage M13.
    Proc Natl Acad Sci U S A. 1984 Jun;81(12):3801-5 PMID: 6427775
  24. Isolation and characterization of pyrimidine mutants of Salmonella typhimurium altered in expression of pyrC, pyrD, and pyrE.
    Can J Microbiol. 1985 Nov;31(11):981-7 PMID: 3004684
  25. The 3'-terminal sequence of Escherichia coli 16S ribosomal RNA: complementarity to nonsense triplets and ribosome binding sites.
    Proc Natl Acad Sci U S A. 1974 Apr;71(4):1342-6 PMID: 4598299
  26. Attenuation control of pyrBI operon expression in Escherichia coli K-12.
    Proc Natl Acad Sci U S A. 1983 Jan;80(2):368-72 PMID: 6300835
  27. Optimal computer folding of large RNA sequences using thermodynamics and auxiliary information.
    Nucleic Acids Res. 1981 Jan 10;9(1):133-48 PMID: 6163133
  28. Cloning and characterization of the prs gene encoding phosphoribosylpyrophosphate synthetase of Escherichia coli.
    Mol Gen Genet. 1985;201(2):269-76 PMID: 3003529
  29. Nucleotide sequence and characterization of the pyrF operon of Escherichia coli K12.
    J Biol Chem. 1987 Jul 25;262(21):10239-45 PMID: 2956254
  30. Vectors with restriction-site banks. I. pJRD158, a 3903-bp plasmid containing 28 unique cloning sites.
    Gene. 1984 Jun;28(3):311-8 PMID: 6086459
  31. Role of translation in the UTP-modulated attenuation at the pyrBI operon of Escherichia coli.
    Mol Gen Genet. 1985;201(2):247-51 PMID: 3003527
  32. Inefficient translation initiation causes premature transcription termination in the lacZ gene.
    Cell. 1986 Mar 14;44(5):711-8 PMID: 3081264
Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00334698
Published
1988-05-00
Pages
287-94
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00334698
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2