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PMID: 2904446 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Alphavirus RNA replicase is located on the cytoplasmic surface of endosomes and lysosomes.

The Journal of cell biology ·Vol. 107 ·No. 6 Pt 1 ·1988-12-00 ·Pages 2075-86

Froshauer S, Kartenbeck J, Helenius A

Abstract

Using morphological and cell biological techniques, we have shown that the RNA replicase of Semliki Forest and Sindbis virus (two closely related alphaviruses) is located in complex ribonucleoprotein structures associated with the cytoplasmic surface of modified secondary lysosomes and endosomes. These nucleoprotein complexes often form a bridge between the membrane of the endocytic vacuole and the rough endoplasmic reticulum where the synthesis of the structural proteins of these viruses occurs. The results suggest that these cytopathic vacuoles constitute sites not only for viral RNA synthesis, but also for translation of structural proteins, and for the assembly of nucleocapsids.

MeSH Terms
Alphavirus/enzymology,growth & development Animals Cell Compartmentation Cell Line Endocytosis Endosomes/enzymology,ultrastructure Fluorescent Antibody Technique Immunohistochemistry Lysosomes/enzymology,ultrastructure Microscopy, Electron Precipitin Tests RNA Nucleotidyltransferases/metabolism RNA, Viral/biosynthesis RNA-Dependent RNA Polymerase/metabolism Ribonucleoproteins/metabolism Vacuoles/enzymology,ultrastructure Virus Replication
Chemicals
RNA, Viral Ribonucleoproteins RNA Nucleotidyltransferases RNA-Dependent RNA Polymerase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Froshauer S
Department of Cell Biology, Yale University School of Medicine, New Haven, Connecticut 06510.
Kartenbeck J
Helenius A
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44 references, click to expand
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.107.6.2075
Published
1988-12-00
Pages
2075-86
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2115628
Subset
IM
Grants
NIGMS NIH HHS · R01 GM 38346-07 · United States
External Links
PubMed source DOI: 10.1083/jcb.107.6.2075 PMC Full Text View journal details
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