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PMID: 290990 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Binding of ribosomes to 5'-terminal leader sequences of eukaryotic messenger RNAs.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 76 ·No. 7 ·1979-07-00 ·Pages 3111-5

Filipowicz W, Haenni AL

Abstract

Tobacco mosaic virus and turnip yellow mosaic virus genome RNAs, which have long (greater than or equal to 69 nucleotides) 5'-terminal leader sequences preceding the AUG initiation codon, can form disome initiation complexes when incubated with a wheat germ extract and sparsomycin. In tobacco mosaic virus RNA, whereas one ribosome in such complexes presumably occupies the AUG-containing initiation site, the other is located within the RNA leader sequence. The results favor a model of eukaryotic initiation in which ribosomes first bind to the 5' end of the mRNA independently of AUG, then advance to the AUG initiation codon, where protein synthesis ensues.

MeSH Terms
Base Sequence Codon/metabolism Models, Biological Mosaic Viruses/metabolism Polyribosomes/metabolism RNA, Messenger/metabolism RNA, Viral/metabolism Ribosomes/metabolism Tobacco Mosaic Virus/metabolism Triticum/metabolism
Chemicals
Codon RNA, Messenger RNA, Viral
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Filipowicz W
Haenni A L
References (32)
32 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.76.7.3111
Published
1979-07-00
Pages
3111-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC383773
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.76.7.3111 PMC Full Text View journal details
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