Home LiteratureArticle Details
PMID: 291015 Published · ppublish English Journal Article

Human T gamma globin chain is a variant of A gamma chain (A gamma Sardinia).

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 76 ·No. 7 ·1979-07-00 ·Pages 3420-4

Saglio G, Ricco G, Mazza U, Camaschella C, Pich PG, Gianni AM, Gianazza E, Righetti PG, Giglioni B, Comi P, Gusmeroli M, Ottolenghi S

Abstract

Isoelectric focusing, cellulose acetate electrophoresis, and carboxymethylcellulose chromatography in the presence of Nonidet P-40 allow the separation of pure gamma chains into two fractions. Amino acid analysis of their cyanogen bromide fragment 3 (gamma CB3) identifies these fractions as the separated G gamma (Gly-136) and A gamma (Ala-136) globin chains. Fingerprint and amino acid analyses of the gamma Tp9 tryptic peptide from the purified A gamma and G gamma fractions from two different patients demonstrate that the commonly occurring gamma Sardinia variant (gamma 75 isoleucine leads to threonine), also known as T gamma chain, has alanine in position 136. From this analysis we suggest that the T gamma gene is an allele of the A gamma locus (A gamma Sardinia) rather than a third gamma locus.

MeSH Terms
Alleles Amino Acid Sequence Detergents Electrophoresis, Cellulose Acetate Globins/genetics,isolation & purification Humans Isoelectric Focusing Mutation Thalassemia/blood
Chemicals
Detergents Globins
Authors & Affiliations
12 authors, click to expand affiliations / ORCID
Saglio G
Ricco G
Mazza U
Camaschella C
Pich P G
Gianni A M
Gianazza E
Righetti P G
Giglioni B
Comi P
Gusmeroli M
Ottolenghi S
References (17)
17 references, click to expand
  1. An improved method for the fingerprinting of human hemoglobin.
    Biochim Biophys Acta. 1961 Apr 1;48:392-6 PMID: 13685867
  2. Abnormal human haemoglobins. I. The comparison of normal human and sickle-cell haemoglobins by fingerprinting.
    Biochim Biophys Acta. 1958 Jun;28(3):539-45 PMID: 13560404
  3. Human globin chain separation by isoelectric focusing.
    J Biochem Biophys Methods. 1979;1(1):45-57 PMID: 551975
  4. G gamma and A gamma globin chains separation and quantitation by isoelectric focusing.
    Biochem Biophys Res Commun. 1979 Mar 15;87(1):1-8 PMID: 454390
  5. The chemical heterogeneity of the fetal hemoglobin in normal newborn infants and in adults.
    Mol Cell Biochem. 1977 Aug 19;17(1):45-55 PMID: 904619
  6. The present status of the heterogeneity of fetal hemoglobin in beta-thalassemia: an attempt to unify some observations in thalassemia and related conditions.
    Ann N Y Acad Sci. 1974;232(0):107-24 PMID: 4528800
  7. Haemoglobin G Copenhagen and haemoglobin J Cambridge. Two new beta-chain variants of haemoglobin A.
    Biochim Biophys Acta. 1967 Jun 27;140(2):231-42 PMID: 6048303
  8. A partial amino acid sequence for sheep haemoblogin A.
    Biochem J. 1967 Apr;103(1):129-40 PMID: 6033754
  9. World-wide occurrence of nonallelic genes for the -chain of human foetal haemoglobin in newborns.
    Nat New Biol. 1972 Dec 27;240(104):273-4 PMID: 4512230
  10. Non-synchronized suppression of postnatal activity in non-allelic genes which synthesize the Ggamma chain in human foetal haemoglobin.
    Nat New Biol. 1973 Jul 18;244(133):89-90 PMID: 4516146
  11. A direct estimate of the number of human gamma-globin genes.
    Cell. 1976 May;8(1):13-8 PMID: 954089
  12. Conformational studies on proteins by isoelectric focusing.
    Ann N Y Acad Sci. 1973 Jun 15;209:198-209 PMID: 4515034
  13. Futher investigations of the gamma-chaina in a ghanaian adult, homozygous for hereditary perisitence of fetal haemoglobin. Isolation of gamma CB-3 peptides and Ggamma:Agamma ratio determination in human Hb E.
    Acta Haematol. 1975;53(5):315-20 PMID: 808072
  14. A new Hb variant: Hb F Sardinia gamma75(E19) isoleucine leads to threonine found in a family with Hb G Philadelphia, beta-chain deficiency and a Lepore-like haemoglobin indistinguishable from Hb A2.
    Acta Haematol. 1975;53(6):347-55 PMID: 808940
  15. Significance of a new type of human fetal hemoglobin carrying a replacement isoleucine replaced by threonine at position 75 )E 19) of the gamma chain.
    Hum Genet. 1976 Jun 29;32(3):305-13 PMID: 939551
  16. Evidence for multiple structural genes for the gamma chain of human fetal hemoglobin.
    Proc Natl Acad Sci U S A. 1968 Jun;60(2):537-44 PMID: 5248810
  17. Abnormal human haemoglobins. Separation and characterization of the alpha and beta chains by chromatography, and the determination of two new variants, hb Chesapeak and hb J (Bangkok).
    J Mol Biol. 1966 Aug;19(1):91-108 PMID: 5967288
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.76.7.3420
Published
1979-07-00
Pages
3420-4
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC383837
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.76.7.3420 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2