Crystal structure of galactan-binding mouse immunoglobulin J539 Fab at 4.5-A resolution.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 76 ·No. 8 ·1979-08-00 ·Pages 4071-4

Navia MA, Segal DM, Padlan EA, Davies DR, Rao N, Rudikoff S, Potter M

Abstract

An electron-density map of the mouse galactan-binding immunoglobulin J539 (IgA2,kappa) Fab has been calculated to a resolution of 4.5 A by the method of heavy atom isomorphous replacement with four derivatives. The map has been interpreted with the aid of a computer program which systematically searched for the best fit between the electron-density map and the known coordinates of individual immunoglobulin domains. The quaternary structure of J539 Fab at this resolution appears similar to that of another mouse immunoglobulin, IgA2,kappa Fab, McPC603. The model coordinates for J539 Fab should allow us to proceed directly to a high-resolution structure determination without further heavy atom isomorphous replacement.

MeSH Terms

Animals Antibody Specificity Crystallography Galactans/immunology Immunoglobulin A Immunoglobulin Fab Fragments Mice Protein Conformation X-Ray Diffraction

Chemicals

Galactans Immunoglobulin A Immunoglobulin Fab Fragments

Authors & Affiliations

7 authors, click to expand affiliations / ORCID

Navia M A

Segal D M

Padlan E A

Davies D R

Rao N

Rudikoff S

Potter M

References (12)

12 references, click to expand

Three-dimensional structure of an intact human immunoglobulin.
Proc Natl Acad Sci U S A. 1977 Nov;74(11):5140-4 PMID: 270751
Crystal structure of the human Fab fragment Kol and its comparison with the intact Kol molecule.
J Mol Biol. 1978 Jun 5;121(4):441-59 PMID: 671546
Crystallographic study of turkey egg-white lysozyme and its complex with a disaccharide.
J Mol Biol. 1977 Jul 5;113(3):555-65 PMID: 886621
Quaternary and tertiary structure of haemerythrin.
Nature. 1975 Oct 30;257(5529):818-21 PMID: 1186872
Structure at 4.5 A resolution of a phosphorylcholine-binding fab.
Nat New Biol. 1973 Oct 10;245(145):165-7 PMID: 4517781
Spectral changes on binding of oligosaccharides to murine immunoglobulin A myeloma proteins.
Biochemistry. 1973 Jul 31;12(16):3039-44 PMID: 4581145
Crystals of phosphorylcholine-binding Fab-fragments from mouse myeloma proteins: preparation and x-ray analysis.
Proc Natl Acad Sci U S A. 1972 Dec;69(12):3689-92 PMID: 4566456
Three-dimensional structure of the Fab' fragment of a human immunoglobulin at 2,8-A resolution.
Proc Natl Acad Sci U S A. 1973 Dec;70(12):3305-10 PMID: 4519624
The three-dimensional structure of a phosphorylcholine-binding mouse immunoglobulin Fab and the nature of the antigen binding site.
Proc Natl Acad Sci U S A. 1974 Nov;71(11):4298-302 PMID: 4530984
The structure of bovine carboxypeptidase B: results at 5-5 Angstrom resolution.
J Mol Biol. 1974 Mar 25;84(1):97-101 PMID: 4830865
Structure of the human antibody molecule Kol (immunoglobulin G1): an electron density map at 5 A resolution.
J Mol Biol. 1976 Jan 25;100(3):257-78 PMID: 1255713
Crystal structure of sickle-cell deoxyhemoglobin at 5 A resolution.
J Mol Biol. 1975 Oct 15;98(1):179-94 PMID: 1195378

Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.76.8.4071

Published

1979-08-00

Pages

4071-4

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC383979

Subset

External Links

PubMed source DOI: 10.1073/pnas.76.8.4071 PMC Full Text View journal details

Analysis Services

Crystal structure of galactan-binding mouse immunoglobulin J539 Fab at 4.5-A resolution.

Contact

WeChat Official Account