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PMID: 2913024 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Antigenically variable Borrelia burgdorferi isolated from cottontail rabbits and Ixodes dentatus in rural and urban areas.

Journal of clinical microbiology ·Vol. 27 ·No. 1 ·1989-01-00 ·Pages 13-20

Anderson JF, Magnarelli LA, LeFebvre RB, Andreadis TG, McAninch JB, Perng GC, Johnson RC

Abstract

Spirochetes were isolated from 71 subadult Ixodes dentatus removed from cottontail rabbits captured in Millbrook, N.Y., and in New York, N.Y. Spirochetes were also cultured from kidney tissues of six rabbits. While all isolates reacted with monoclonal antibody H9724, which identifies the spirochetes as borreliae, more than half did not bind with antibody H5332 and even fewer reacted with H3TS, both of which were produced to outer surface protein A of Borrelia burgdorferi. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis protein profiles of three isolates differed from one another and from all previously characterized B. burgdorferi strains from humans, ticks, and wildlife in North America. The 12 periplasmic flagella that originated subterminally from each pointed end of a rabbit Borellia isolate contrasted with the 11 or fewer flagella for B. burgdorferi reported previously from North America. Although DNA homology and restriction endonuclease analysis also revealed differences among a rabbit kidney isolate, an I. dentatus isolate, and B. burgdorferi B31, similarities were sufficient to lead us to conclude that the borreliae in rabbits and I. dentatus are B. burgdorferi. Enzyme-linked immunosorbent assay titers of sera from humans with diagnosed Lyme disease to rabbit tick B. burgdorferi were often similar to one another and to those recorded for a reference B. burgdorferi strain.

MeSH Terms
Animals Antigenic Variation Antigens, Bacterial/analysis Blotting, Western Borrelia/classification,immunology,isolation & purification,ultrastructure DNA, Bacterial/analysis Electrophoresis, Polyacrylamide Gel Enzyme-Linked Immunosorbent Assay Lyme Disease/epidemiology,microbiology,veterinary Microscopy, Electron New York New York City Nucleic Acid Hybridization Rabbits Restriction Mapping Serotyping Ticks/microbiology
Chemicals
Antigens, Bacterial DNA, Bacterial
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Anderson J F
Department of Entomology, Connecticut Agricultural Experiment Station, New Haven 06504.
Magnarelli L A
LeFebvre R B
Andreadis T G
McAninch J B
Perng G C
Johnson R C
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Article Info
Journal
Journal of clinical microbiology
Abbr.
J Clin Microbiol
ISSN
0095-1137
DOI
10.1128/jcm.27.1.13-20.1989
Published
1989-01-00
Pages
13-20
Language
English
Region
United States
NLM ID
7505564
PMCID
PMC267224
Subset
IM
Grants
NIAID NIH HHS · AI 18153 · United States
External Links
PubMed source DOI: 10.1128/jcm.27.1.13-20.1989 PMC Full Text View journal details
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