Translation and processing of Bacillus amyloliquefaciens extracellular RNase.

Journal of bacteriology ·Vol. 171 ·No. 2 ·1989-02-00 ·Pages 1185-7

Paddon CJ, Vasantha N, Hartley RW

Abstract

Bacillus amyloliquefaciens extracellular RNase has been previously cloned and expressed in Bacillus subtilis. Site-specific mutagenesis experiments have identified codon -39 as the start site of translation. We have determined the primary signal peptide cleavage site of preprobarnase and propose a pathway for the conversion of probarnase to mature barnase.

MeSH Terms

Amino Acid Sequence Bacillus/enzymology,genetics Base Sequence Codon/genetics Genes Genes, Bacterial Molecular Sequence Data Mutation Protein Biosynthesis Protein Processing, Post-Translational Protein Sorting Signals/genetics Ribonucleases/genetics

Chemicals

Codon Protein Sorting Signals Ribonucleases

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Paddon C J

Laboratory of Cellular and Developmental Biology, National Institute of Diabetes and Digestive and Kidney Diseases, Bethesda, Maryland 20892.

Vasantha N

Hartley R W

References (10)

10 references, click to expand

"Western blotting": electrophoretic transfer of proteins from sodium dodecyl sulfate--polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A.
Anal Biochem. 1981 Apr;112(2):195-203 PMID: 6266278
Barnase and barstar. Expression of its cloned inhibitor permits expression of a cloned ribonuclease.
J Mol Biol. 1988 Aug 20;202(4):913-5 PMID: 3050134
Cloning, sequencing and transcription of an inactivated copy of Bacillus amyloliquefaciens extracellular ribonuclease (barnase).
Gene. 1985;40(2-3):231-9 PMID: 3007290
Use of plasmid pTV1 in transposon mutagenesis and gene cloning in Bacillus amyloliquefaciens.
Plasmid. 1986 Jul;16(1):45-51 PMID: 3016781
Cloning in single-stranded bacteriophage as an aid to rapid DNA sequencing.
J Mol Biol. 1980 Oct 25;143(2):161-78 PMID: 6260957
Expression of Bacillus amyloliquefaciens extracellular ribonuclease (barnase) in Escherichia coli following an inactivating mutation.
Gene. 1987;53(1):11-9 PMID: 3297926
Site-directed mutagenesis of cytochrome c shows that an invariant Phe is not essential for function.
Nature. 1985 Jan 10-18;313(5998):152-4 PMID: 2981414
Amino-acid sequence of extracellular ribonuclease (barnase) of Bacillus amyloliquefaciens.
Nat New Biol. 1972 Jan 5;235(53):15-6 PMID: 4553460
Production and purification of the extracellular ribonuclease of Bacillus amyloliquefaciens (barnase) and its intracellular inhibitor (barstar). II. Barstar.
Prep Biochem. 1972;2(3):243-50 PMID: 5031050
Fusion of pro region of subtilisin to staphylococcal protein A and its secretion by Bacillus subtilis.
Gene. 1986;49(1):23-8 PMID: 3106154

Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.171.2.1185-1187.1989

Published

1989-02-00

Pages

1185-7

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC209718

Subset

Databases

GENBANK

M14442

External Links

PubMed source DOI: 10.1128/jb.171.2.1185-1187.1989 PMC Full Text View journal details

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Translation and processing of Bacillus amyloliquefaciens extracellular RNase.

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