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PMID: 2919175 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Targeting of a chromosomal protein to the nucleus and to lampbrush chromosome loops.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 86 ·No. 4 ·1989-02-00 ·Pages 1269-72

Roth MB, Gall JG

Abstract

We have isolated a cDNA clone (SE5A) that encodes a protein on lampbrush chromosome loops of the newt Notophthalmus. In vitro-synthesized transcripts of this clone were injected into Xenopus oocytes, where they were efficiently translated. Most of the translated protein was imported into the oocyte nucleus, and some of it appeared on the chromosome loops. The translation product must contain information that permits its appropriate targeting first to the nucleus and then to the chromosome loops.

MeSH Terms
Amino Acid Sequence Animals Chromosome Mapping DNA/genetics Female Genes Molecular Sequence Data Nuclear Proteins/genetics Oocytes/metabolism Plasmids Salamandridae/genetics Transcription, Genetic Xenopus laevis
Chemicals
Nuclear Proteins DNA
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Roth M B
Department of Embryology, Carnegie Institution of Washington, Baltimore, MD 21210.
Gall J G
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21 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.86.4.1269
Published
1989-02-00
Pages
1269-72
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC286669
Subset
IM
Grants
NIGMS NIH HHS · GM 33397 · United States
External Links
PubMed source DOI: 10.1073/pnas.86.4.1269 PMC Full Text View journal details
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