Home LiteratureArticle Details
PMID: 291949 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Uroporphyrinogen I synthase induction in normal human bone marrow cultures: an early and quantitative response of erythroid differentiation.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 76 ·No. 10 ·1979-10-00 ·Pages 5321-5

Sassa S, Urabe A

Abstract

Uroporphyrinogen I (URO) synthase [porphobilinogen ammonia-lyase (polymerizing), EC 4.3.1.8] activity increased when cultures of normal human bone marrow cells were incubated with erythropoietin. The increase of URO synthase activity was a linear function of erythropoietin concentration in the culture medium and was proportional to the extent of heme synthesis as determined by 55Fe incorporation into heme. The onset of the increase in URO synthase activity, which occurred on day 4 of incubation with erythropoietin, preceded by 3 days the appearance of hemoglobin in colonies derived from erythroid progenitor cells. These results indicate that induction of URO synthase in normal human bone marrow cells incubated with erythropoietin is an early event and that URO synthase activity is a useful quantitative index of erythroid differentiation.

MeSH Terms
Adult Ammonia-Lyases/biosynthesis Bone Marrow/drug effects,enzymology,physiology Cell Differentiation Cells, Cultured Enzyme Induction/drug effects Erythropoiesis Erythropoietin/pharmacology Humans Hydroxymethylbilane Synthase/biosynthesis Kinetics Spectrometry, Fluorescence
Chemicals
Erythropoietin Hydroxymethylbilane Synthase Ammonia-Lyases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Sassa S
Urabe A
References (20)
20 references, click to expand
  1. Deficient heme synthesis as the cause of noninducibility of hemoglobin synthesis in a Friend erythroleukemia cell line.
    Cell. 1979 Feb;16(2):415-23 PMID: 287566
  2. Increase in globin chains and globin mRNA in erythroleukemia cells in response to hemin.
    Arch Biochem Biophys. 1977 Feb;179(1):106-12 PMID: 265145
  3. Heme biosynthesis in Friend erythroleukemia cells: control by ferrochelatase.
    Proc Natl Acad Sci U S A. 1979 Feb;76(2):833-6 PMID: 284406
  4. Hemin control of heme biosynthesis in mouse Friend virus-transformed erythroleukemia cells in culture.
    J Biol Chem. 1978 Aug 10;253(15):5402-6 PMID: 276528
  5. Levels of delta-aminolevulinate dehydratase, uroporphyrinogen-I synthase, and protoporphyrin IX in erythrocytes from anemic mutant mice.
    Proc Natl Acad Sci U S A. 1977 Mar;74(3):1181-4 PMID: 265562
  6. Induction of aminolevulinate synthase and porphyrins in cultured liver cells maintained in chemically defined medium. Permissive effects of hormones on induction process.
    J Biol Chem. 1977 Apr 10;252(7):2428-36 PMID: 321458
  7. Enhancement of erythroid colony growth in culture by hemin.
    Exp Hematol. 1979 Jan;7(1):11-6 PMID: 428474
  8. Circulating erythropoietic precursors assessed in culture: characterization in normal men and patients with hemoglobinopathies.
    Blood. 1977 Dec;50(6):1081-92 PMID: 922159
  9. Human marrow cells capable of erythropoietic differentiation in vitro: definition of three erythroid colony responses.
    Blood. 1977 Jun;49(6):855-64 PMID: 861374
  10. Sequential induction of heme pathway enzymes during erythroid differentiation of mouse Friend leukemia virus-infected cells.
    J Exp Med. 1976 Feb 1;143(2):305-15 PMID: 1249519
  11. Induction of globin mRNA accumulation by hemin in cultured erythroleukemic cells.
    Cell. 1976 Aug;8(4):513-20 PMID: 954104
  12. Sequential induction of enzymes in the heme biosynthetic pathway during erythroid differentiation.
    Nihon Ketsueki Gakkai Zasshi. 1975 Dec;38(6):715-22 PMID: 1243796
  13. Linear sequential arrangement of genes for the biosynthetic pathway of protoheme in Staphylococcus aureus.
    Proc Natl Acad Sci U S A. 1968 Dec;61(4):1392-8 PMID: 4236816
  14. A microassay for uroporphyrinogen I synthase, one of three abnormal enzyme activities in acute intermittent porphyria, and its application to the study of the genetics of this disease.
    Proc Natl Acad Sci U S A. 1974 Mar;71(3):732-6 PMID: 4522787
  15. Erythropietic colonies in cultures of human marrow.
    Blood. 1974 Nov;44(5):659-69 PMID: 4472812
  16. Erythroid colony formation in cultures of mouse and human bone marrow: analysis of the requirement for erythropoietin by gel filtration and affinity chromatography on agarose-concanavalin A.
    J Cell Physiol. 1974 Apr;83(2):309-20 PMID: 4822532
  17. Studies in lead poisoning. I. Microanalysis of erythrocyte protoporphyrin levels by spectrophotometry in the detection of chronic lead intoxication in the subclinical range.
    Biochem Med. 1973 Aug;8(1):135-48 PMID: 4744314
  18. Human marrow erythropoiesis in culture. I. Characterization of methylcellulose colony assay.
    Blood. 1976 Sep;48(3):407-17 PMID: 953362
  19. Erythroid progenitors in mouse bone marrow detected by macroscopic colony formation in culture.
    Exp Hematol. 1975 Jan;3(1):32-43 PMID: 1157835
  20. The activities of three enzymes of haem synthesis during hepatic erythropoiesis in the mouse embryo.
    J Embryol Exp Morphol. 1971 Oct;26(2):313-22 PMID: 5157351
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.76.10.5321
Published
1979-10-00
Pages
5321-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC413134
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.76.10.5321 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2