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PMID: 291988 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

DNA restriction endonuclease analysis for localization of human beta- and delta-globin genes on chromosome 11.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 76 ·No. 9 ·1979-09-00 ·Pages 4563-5

Scott AF, Phillips JA, Migeon BR

Abstract

DNA from a clone of a mouse-human hybrid that retained a human chromosome consisting of the major part of chromosome 11 and region q25-26-qter of the X chromosome was digested with various restriction endonucleases, subjected to electrophoresis in agarose gels, and transferred to nitrocellulose filters. The restriction digest pattern of the clone, when hybridized with a 32P-labeled plasmid fragment containing human beta-globin gene sequences, was a composite of the normal human and mouse (A9) patterns. When back-selected in 6-thioguanine to eliminate the 11 translocation chromosome, the hybrid cells showed only the A9 restriction pattern. These results substantiate the localization of beta- and delta-globin genes to human chromosome 11 and exclude the region 11q23-qter as the site.

MeSH Terms
Animals Chromosome Mapping Chromosomes, Human, 6-12 and X DNA Restriction Enzymes/metabolism Globins/genetics Humans Hybrid Cells Karyotyping Mice Translocation, Genetic
Chemicals
Globins DNA Restriction Enzymes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Scott A F
Phillips J A
Migeon B R
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22 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.76.9.4563
Published
1979-09-00
Pages
4563-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC411618
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.76.9.4563 PMC Full Text View journal details
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