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PMID: 2921241 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Identification and sequence analysis of two related flagellin genes in Rhizobium meliloti.

Journal of bacteriology ·Vol. 171 ·No. 3 ·1989-03-00 ·Pages 1467-75

Pleier E, Schmitt R

Abstract

The genomic region that codes for the flagellin subunits of the complex flagellar filaments of Rhizobium meliloti was cloned and sequenced. Two structural genes, flaA and flaB, that encode 395- and 396-amino-acid polypeptides, respectively, were identified. These exhibit 87% sequence identity. The amino acid sequences of tryptic peptides suggest that both of these subunit proteins are represented in the flagellar filaments. The N-terminal methionine was absent from the mature flagellin subunits. Their derived primary structures show almost no relationship to flagellins from Escherichia coli, Salmonella typhimurium, or Bacillus subtilis but exhibit up to 60% similarity to the N- and C-terminal portions of flagellin from Caulobacter crescentus. It is suggested that the complex flagellar filaments of R. meliloti are unique in being assembled from heterodimers of two related flagellin subunits. The tandemly arranged flagellin genes were shown to be transcribed separately from unusual promoter sequences.

MeSH Terms
Amino Acid Sequence Bacterial Proteins/genetics Base Sequence Chromosomes, Bacterial Cloning, Molecular Codon/genetics Flagellin/genetics,isolation & purification Genes Genes, Bacterial Genetic Vectors Macromolecular Substances Molecular Sequence Data Nucleic Acid Hybridization Recombinant Proteins/isolation & purification Restriction Mapping Rhizobium/genetics Sequence Homology, Nucleic Acid
Chemicals
Bacterial Proteins Codon Macromolecular Substances Recombinant Proteins Flagellin
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Pleier E
Lehrstuhl für Genetik, Universität Regensburg, Federal Republic of Germany.
Schmitt R
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.171.3.1467-1475.1989
Published
1989-03-00
Pages
1467-75
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC209768
Subset
IM
Databases
GENBANK
J04231, M24526
External Links
PubMed source DOI: 10.1128/jb.171.3.1467-1475.1989 PMC Full Text View journal details
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