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PMID: 2930494 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Differences in phorbol-ester-induced down-regulation of protein kinase C between cell lines.

The Biochemical journal ·Vol. 257 ·No. 3 ·1989-02-01 ·Pages 905-11

Adams JC, Gullick WJ

Abstract

Down-regulation of protein kinase C induced by 12-O-tetradecanoylphorbol 13-acetate (TPA) was examined in Swiss 3T3, V79, MDBK and C6 cells by Western blotting. Variations in the rate of down-regulation caused by treatment with 100 nM-TPA were observed; TPA treatment for 5 h caused maximal down-regulation in V79 cells, whereas TPA treatment for 10 h or 30 h was needed for maximal down-regulation of protein kinase C in MDBK or Swiss 3T3 cells respectively. The decrease in amount of immunologically detectable protein kinase C was 30% in MDBK cells and 100% in V79 and Swiss 3T3 cells. MDBK and C6 cells could be completely depleted of protein kinase C by treatment with 250 nM-TPA. In C6 cells, after treatment with 500 nM-TPA, an 80% loss of protein kinase C was seen over 10 h. Measurement of the numbers of phorbol-ester-binding sites remaining in each cell line when protein kinase C was maximally down-regulated indicated that in MDBK and Swiss 3T3 cells loss of phorbol-ester-binding sites paralleled loss of protein kinase C, whereas in V79 and C6 cells no such correlation was observed.

MeSH Terms
Animals Binding Sites Cell Line Cricetinae Phorbol 12,13-Dibutyrate/metabolism Protein Kinase C/metabolism Tetradecanoylphorbol Acetate/pharmacology Tumor Cells, Cultured
Chemicals
Phorbol 12,13-Dibutyrate Protein Kinase C Tetradecanoylphorbol Acetate
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Adams J C
Chester Beatty Research Laboratories, Institute of Cancer Research, London, U.K.
Gullick W J
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj2570905
Published
1989-02-01
Pages
905-11
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1135673
Subset
IM
External Links
PubMed source DOI: 10.1042/bj2570905 PMC Full Text View journal details
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