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PMID: 2930822 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

The spectrin network as a barrier to lateral diffusion in erythrocytes. A percolation analysis.

Biophysical journal ·Vol. 55 ·No. 1 ·1989-01-00 ·Pages 21-8

Saxton MJ

Abstract

The spectrin network on the cytoplasmic surface of an erythrocyte can be modeled as a triangular lattice of spectrin tetramers (Tsuji, A., and S. Ohnishi, 1986. Biochemistry. 25:6133-6139). The tetramers act as barriers to protein diffusion, while dissociated dimer pairs, single dimers, and missing tetramers do not. Diffusion in the presence of these barriers is shown to be equivalent to bond percolation on the honeycomb lattice. Monte Carlo calculations for this system then yield the relative diffusion constant of a mobile integral protein as a function of the fraction of spectrin tetramers. At high concentrations of spectrin tetramer, long-range diffusion is blocked, but short-range diffusion is still possible. Monte Carlo calculations yield the average distance over which short-range diffusion can occur, as a function of the fraction of spectrin tetramers. Applications to erythrocyte development and hereditary hemolytic anemia are discussed.

MeSH Terms
Algorithms Diffusion Erythrocyte Membrane/metabolism Humans Kinetics Macromolecular Substances Models, Theoretical Monte Carlo Method Protein Conformation Spectrin/metabolism
Chemicals
Macromolecular Substances Spectrin
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Saxton M J
Department of Agronomy and Range Science, University of California, Davis 95616.
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50 references, click to expand
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Article Info
Journal
Biophysical journal
Abbr.
Biophys J
ISSN
0006-3495
DOI
10.1016/S0006-3495(89)82776-6
Published
1989-01-00
Pages
21-8
Language
English
Region
United States
NLM ID
0370626
PMCID
PMC1330439
Subset
IM
Grants
NIGMS NIH HHS · 1 R01 GM38133-01A1 · United States
External Links
PubMed source DOI: 10.1016/S0006-3495(89)82776-6 PMC Full Text View journal details
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