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PMID: 29330721 Published · ppublish English Journal Article

Transcriptomic analysis and discovery of genes in the response of Arachis hypogaea to drought stress.

Molecular biology reports ·Vol. 45 ·No. 2 ·2018-04-00 ·Pages 119-131

Zhao X, Li C, Wan S, Zhang T, Yan C, Shan S

Abstract

The peanut (Arachis hypogaea) is an important crop species that is threatened by drought stress. The genome sequences of peanut, which was officially released in 2016, may help explain the molecular mechanisms that underlie drought tolerance in this species. We report here a gene expression profiling of A. hypogaea to gain a global view of its drought resistance. Using whole-transcriptome sequencing, we analysed differential gene expression in response to drought stress in the drought-resistant peanut cultivar J11. Pooled samples obtained at 6, 12, 18, 24, and 48 h were compared with control samples at 0 h. In total, 51,554 genes were found, including 49,289 known genes and 2265 unknown genes. We identified 224 differentially expressed transcription factors, 296,335 SNPs and 28,391 InDELs. In addition, we detected significant differences in the gene expression profiles of the treatment and control groups. After comparing the two groups, 4648 genes were identified. An in-depth analysis of the data revealed that a large number of genes were associated with drought stress, including transcription factors and genes involved in photosynthesis-antenna proteins, carbon metabolism and the citrate cycle. The results of this study provide insights into the diverse mechanisms that underlie the successful establishment of drought resistance in the peanut, thereby facilitating the identification of important genes in the peanut related to drought management. Transcriptome analysis based on RNA-Seq is a powerful approach for gene discovery and molecular marker development for this species.

Keywords
Arachis hypogaea Drought stress RNA-Seq Root genes
MeSH Terms
Arachis/genetics Droughts Gene Expression Profiling/methods Gene Expression Regulation, Plant/genetics Genes, Plant/genetics Plant Roots Stress, Physiological/genetics Transcriptome/genetics Whole Exome Sequencing/methods
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Zhao Xiaobo
Laboratory of Genetics and Breeding, Shandong Peanut Research Institute, Qingdao, 266100, Shandong Province, People's Republic of China.
Li Chunjuan
Laboratory of Genetics and Breeding, Shandong Peanut Research Institute, Qingdao, 266100, Shandong Province, People's Republic of China.
Wan Shubo
Shandong Academy of Agricultural Sciences, Jinan, 250100, Shandong Province, People's Republic of China.
Zhang Tingting
Laboratory of Genetics and Breeding, Shandong Peanut Research Institute, Qingdao, 266100, Shandong Province, People's Republic of China.
Yan Caixia
Laboratory of Genetics and Breeding, Shandong Peanut Research Institute, Qingdao, 266100, Shandong Province, People's Republic of China.
Shan Shihua
Laboratory of Genetics and Breeding, Shandong Peanut Research Institute, Qingdao, 266100, Shandong Province, People's Republic of China. [email protected].
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Article Info
Journal
Molecular biology reports
Abbr.
Mol Biol Rep
ISSN
1573-4978
DOI
10.1007/s11033-018-4145-4
Email
[email protected]
Published
2018-04-00
Epub
2018-00-12
Pages
119-131
Language
English
Region
Netherlands
NLM ID
0403234
Subset
IM
Grants
International Science and Technology Cooperation Program of China · 2015DFA31190
Young Scholars Fundation of Shandong Academy of Agricultural Sciences · 2016-YQN16
National Science and Technology Support Program · 2014BAD11B00
External Links
PubMed source DOI: 10.1007/s11033-018-4145-4 View journal details
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