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PMID: 2938521 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Sequential detection of antigens in Western blots with differently colored products.

Analytical biochemistry ·Vol. 152 ·No. 2 ·1986-02-01 ·Pages 211-4

Theisen N, Lohoff ES, von Figura K, Hasilik A

Abstract

A reliable sequential application of peroxidase and alkaline phosphatase-conjugated second antibodies to detection of two different antigens in Western blots is described. Deposits of differently colored products formed allow identification of several antigens on a single blot.

MeSH Terms
Alkaline Phosphatase Antigens/isolation & purification Clathrin/immunology,isolation & purification Collodion Color Electrophoresis, Polyacrylamide Gel Hexosaminidases/immunology,isolation & purification Humans Immunoenzyme Techniques Isoenzymes/immunology,isolation & purification Peroxidases Protein Conformation beta-N-Acetylhexosaminidases
Chemicals
Antigens Clathrin Isoenzymes Collodion Peroxidases Alkaline Phosphatase Hexosaminidases beta-N-Acetylhexosaminidases
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Theisen N
Lohoff E S
von Figura K
Hasilik A
Article Info
Journal
Analytical biochemistry
Abbr.
Anal Biochem
ISSN
0003-2697
DOI
10.1016/0003-2697(86)90399-4
Published
1986-02-01
Pages
211-4
Language
English
Region
United States
NLM ID
0370535
Subset
IM
External Links
PubMed source DOI: 10.1016/0003-2697(86)90399-4 View journal details
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