Home LiteratureArticle Details
PMID: 2945079 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Derepression of single-stranded DNA-binding protein genes on plasmids derepressed for conjugation, and complementation of an E. coli ssb- mutation by these genes.

Molecular & general genetics : MGG ·Vol. 204 ·No. 3 ·1986-09-00 ·Pages 410-6

Golub EI, Low KB

Abstract

Plasmid single-stranded DNA-binding protein genes complement the E. coli ssb-1 mutation, and partially restore capacity for DNA synthesis, DNA repair (direct role as well as role in SOS induction) and general recombination. Plasmid mutants derepressed for fertility derived from R1, R64 and R222 show a higher level of complementation compared to the parental repressed plasmids. Derepressed mutants of R222 synthesize more RNA which hybridizes with the ssb gene of the F factor than does the original R222 plasmid. This indicates that plasmid ssb genes are regulated coordinately with fertility genes.

MeSH Terms
Bacteriophage lambda/drug effects,genetics Conjugation, Genetic DNA, Single-Stranded/metabolism DNA-Binding Proteins/genetics Escherichia coli/genetics,radiation effects Genes Genes, Bacterial Genetic Complementation Test Genotype Mitomycin Mitomycins/pharmacology Mutation Nucleic Acid Hybridization Plasmids Ultraviolet Rays
Chemicals
DNA, Single-Stranded DNA-Binding Proteins Mitomycins Mitomycin
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Golub E I
Low K B
References (24)
24 references, click to expand
  1. DNA repair in E. coli strains deficient in single-strand DNA binding protein.
    Mol Gen Genet. 1981;183(2):341-7 PMID: 6276685
  2. Mutant single-strand binding protein of Escherichia coli: genetic and physiological characterization.
    J Bacteriol. 1979 Oct;140(1):14-9 PMID: 227832
  3. The conjugation system of F-like plasmids.
    Annu Rev Genet. 1980;14:41-76 PMID: 6111288
  4. The generation of a ColE1-Apr cloning vehicle which allows detection of inserted DNA.
    Mol Gen Genet. 1975 Dec 30;142(3):239-49 PMID: 1045010
  5. A simple method for extraction of RNA from E. coli utilizing diethyl pyrocarbonate.
    Anal Biochem. 1970 Feb;33(2):459-63 PMID: 4910776
  6. An Escherichia coli mutant defective in single-strand binding protein is defective in DNA replication.
    Proc Natl Acad Sci U S A. 1979 Apr;76(4):1702-5 PMID: 221903
  7. Detection of transcribable recombination products following conjugation in rec+, reCB- and recC-strains of Escherichia coli K12.
    J Mol Biol. 1974 Mar 15;83(4):447-57 PMID: 4598358
  8. Indirect stimulation of genetic recombination.
    Proc Natl Acad Sci U S A. 1983 Mar;80(5):1401-5 PMID: 6219392
  9. Effects of the ssb-1 and ssb-113 mutations on survival and DNA repair in UV-irradiated delta uvrB strains of Escherichia coli K-12.
    J Bacteriol. 1982 Jul;151(1):186-92 PMID: 7045074
  10. F sex factor of Escherichia coli K-12 codes for a single-stranded DNA binding protein.
    Proc Natl Acad Sci U S A. 1983 Jul;80(14):4422-6 PMID: 6308624
  11. Genetic recombination of bacterial plasmid DNA: effect of RecF pathway mutations on plasmid recombination in Escherichia coli.
    J Bacteriol. 1985 Sep;163(3):1060-6 PMID: 2993230
  12. Isolation and characterization of Tn5 insertion mutations in the lexA gene of Escherichia coli.
    J Bacteriol. 1983 Mar;153(3):1368-78 PMID: 6298183
  13. Pedigrees of some mutant strains of Escherichia coli K-12.
    Bacteriol Rev. 1972 Dec;36(4):525-57 PMID: 4568763
  14. A deoxythymidine kinase deficient mutant of Escherichia coli. II. Mapping and transduction studies with phage phi 80.
    Genetics. 1967 Nov;57(3):643-54 PMID: 4868676
  15. Mating signal and DNA penetration deficiency in conjugation between male Escherichia coli and minicells.
    Proc Natl Acad Sci U S A. 1975 Sep;72(9):3721-5 PMID: 1059160
  16. General method for isolating de-repressed bacterial sex factors.
    Nature. 1968 Aug 24;219(5156):869-70 PMID: 4876938
  17. F sex factor encodes a single-stranded DNA binding protein (SSB) with extensive sequence homology to Escherichia coli SSB.
    Proc Natl Acad Sci U S A. 1983 Sep;80(18):5480-4 PMID: 6351061
  18. A DNA primase specified by I-like plasmids.
    Proc Natl Acad Sci U S A. 1979 Aug;76(8):3632-6 PMID: 386343
  19. Transduction versus "conjuduction": evidence for multiple roles for exonuclease V in genetic recombination in Escherichia coli.
    Cold Spring Harb Symp Quant Biol. 1979;43 Pt 2:1043-7 PMID: 158457
  20. THE ABORTIVE REPLICATION OF PhiX174 DNA IN A RECOMBINATION-DEFICIENT MUTANT OF Escherichia coli.
    Proc Natl Acad Sci U S A. 1967 Mar;57(3):813-20 PMID: 16591535
  21. Cell-free coupled transcription-translation system for investigation of linear DNA segments.
    Proc Natl Acad Sci U S A. 1980 Dec;77(12):7029-33 PMID: 6261235
  22. Conjugative plasmids of enteric bacteria from many different incompatibility groups have similar genes for single-stranded DNA-binding proteins.
    J Bacteriol. 1985 Apr;162(1):235-41 PMID: 3884590
  23. Specificity of mutagenesis resulting from the induction of the SOS system in the absence of mutagenic treatment.
    Cell. 1984 Jun;37(2):675-82 PMID: 6373019
  24. Effect of ssbA1 and lexC113 mutations on lambda prophage induction, bacteriophage growth, and cell survival.
    J Bacteriol. 1980 Aug;143(2):887-96 PMID: 6451615
Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00331017
Published
1986-09-00
Pages
410-6
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
Grants
NIAID NIH HHS · AI20570 · United States
NCI NIH HHS · CA06519 · United States
External Links
PubMed source DOI: 10.1007/BF00331017
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2