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PMID: 2945893 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Direct T helper-B cell interactions induce an early B cell activation antigen.

The Journal of experimental medicine ·Vol. 164 ·No. 5 ·1986-11-01 ·Pages 1760-72

Crow MK, Jover JA, Friedman SM

Abstract

We have explored the consequences for the B cell of cognate interaction with T cells. Early expression of the B cell-restricted cell surface activation antigen, BLAST-2, has been used as an assay system to measure direct T-B cell collaboration. BLAST-2 is preferentially expressed by allogenic B cells cultured with MHC class II antigen-restricted Th clone cells matched to the DR specificity of the target B cells. B cells cultured with DR-mismatched allospecific Th cells express minimal BLAST-2. Th cell-induced BLAST-2 expression appears to be accessory cell independent and occurs as early as 8 h after initiation of culture, with peak expression at 18 h. Direct T-B cell contact, rather than Th-derived lymphokines, provides the most efficient stimulus for BLAST-2 expression. Crosslinking of sIg on B cells is a poor stimulus for BLAST-2 expression. The BLAST-2 assay permits the evaluation of early events associated with B cell activation through cognate interactions, and may facilitate subsequent studies of the mechanism of B cell differentiation.

MeSH Terms
Antibodies, Anti-Idiotypic/immunology Antigen-Presenting Cells/physiology Antigens, Differentiation, B-Lymphocyte Antigens, Surface/biosynthesis B-Lymphocytes/immunology HLA-DR Antigens/immunology Humans Kinetics Lymphocyte Activation Lymphokines/physiology T-Lymphocytes, Helper-Inducer/immunology Trinitrobenzenes/immunology
Chemicals
Antibodies, Anti-Idiotypic Antigens, Differentiation, B-Lymphocyte Antigens, Surface HLA-DR Antigens Lymphokines Trinitrobenzenes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Crow M K
Jover J A
Friedman S M
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28 references, click to expand
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Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.164.5.1760
Published
1986-11-01
Pages
1760-72
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2188453
Subset
IM
Grants
NIAID NIH HHS · AI-18263 · United States
NIADDK NIH HHS · AM-01523 · United States
External Links
PubMed source DOI: 10.1084/jem.164.5.1760 PMC Full Text View journal details
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