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PMID: 2947040 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Perfusion cuvette for the simultaneous measurement of mechanical, optical and energetic parameters of skinned muscle fibres.

Pflugers Archiv : European journal of physiology ·Vol. 407 ·No. 5 ·1986-11-00 ·Pages 552-7

Güth K, Wojciechowski R

Abstract

A micro cuvette is described, which allows to measure mechanical, optical and energetic parameters of single skinned muscle fibres or small fibre bundles. Two examples for possible applications are given: The measurement of ATPase activity of single muscle fibres. The detection of fluorescence changes due to Ca binding to fluorescence labeled TnC, which was incorporated into small bundles of rabbit psoas fibres before the experiment.

MeSH Terms
Adenosine Triphosphatases/analysis Animals Biomechanical Phenomena Fluorescence Muscles/enzymology,physiology Optics and Photonics Photometry Physiology/instrumentation Rabbits Transducers
Chemicals
Adenosine Triphosphatases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Güth K
Wojciechowski R
References (7)
7 references, click to expand
  1. Tension responses to sudden length change in stimulated frog muscle fibres near slack length.
    J Physiol. 1977 Jul;269(2):441-515 PMID: 302333
  2. Stiffness and tension during and after sudden length changes of glycerinated single insect fibrillar muscle fibres.
    Biophys Struct Mech. 1979 Aug;5(4):255-76 PMID: 114244
  3. Calculator programs for computing the composition of the solutions containing multiple metals and ligands used for experiments in skinned muscle cells.
    J Physiol (Paris). 1979;75(5):463-505 PMID: 533865
  4. Polarization of tryptophan fluorescence measurements in muscle. A re-evaluation.
    Biophys Struct Mech. 1980;6(2):81-93 PMID: 7388126
  5. ATPase activity in rapidly activated skinned muscle fibres.
    Pflugers Arch. 1980 Sep;387(2):167-73 PMID: 6448985
  6. Force and force transients in skeletal muscle fibres of the frog skinned by freeze-drying.
    Pflugers Arch. 1983 Jun 1;397(4):272-6 PMID: 6412211
  7. Low Ca2+ impedes cross-bridge detachment in chemically skinned Taenia coli.
    Nature. 1982 Dec 23;300(5894):775-6 PMID: 6217425
Article Info
Journal
Pflugers Archiv : European journal of physiology
Abbr.
Pflugers Arch
ISSN
0031-6768
DOI
10.1007/BF00657515
Published
1986-11-00
Pages
552-7
Language
English
Region
Germany
NLM ID
0154720
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00657515 View journal details
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