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PMID: 2950121 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Phosphomannosyl-derivatized beads detect a receptor involved in lymphocyte homing.

The Journal of cell biology ·Vol. 104 ·No. 3 ·1987-03-00 ·Pages 713-23

Yednock TA, Stoolman LM, Rosen SD

Abstract

Recirculating lymphocytes initiate extravasation from the blood stream by binding to specialized high endothelial venules (HEV) within peripheral lymph nodes (PN) and other secondary lymphoid organs. We have previously reported that lymphocyte attachment to PN HEV is selectively inhibited by mannose-6-phosphate (M6P) and related carbohydrates (Stoolman, L. M., T. S. Tenforde, and S. D. Rosen, 1984, J. Cell Biol., 99:1535-1540). In the present study, we employ a novel cell-surface probe consisting of fluorescent beads derivatized with PPME, a M6P-rich polysaccharide. PPME beads directly identify a carbohydrate-binding receptor on the surface of mouse lymphocytes. In every way examined, lymphocyte attachment to PPME beads (measured by flow cytofluorometry) mimics the interaction of lymphocytes with PN HEV (measured in the Stamper-Woodruff in vitro assay): both interactions are selectively inhibited by the same panel of structurally related carbohydrates, are calcium-dependent, and are sensitive to mild treatment of the lymphocytes with trypsin. In addition, thymocytes and a thymic lymphoma, S49, bind poorly to PPME beads in correspondence to their weak ability to bind to HEV. When the S49 cell line was subjected to a selection procedure with PPME beads, the ability of the cells to bind PPME beads, as well as their ability to bind to PN HEV, increased six- to eightfold. We conclude that a carbohydrate-binding receptor on mouse lymphocytes, detected by PPME beads, is involved in lymphocyte attachment to PN HEV.

MeSH Terms
Animals Carrier Proteins/physiology Cell Adhesion Cell Line Cell Membrane/physiology Cell Movement Flow Cytometry Hexosephosphates/metabolism Lymphocytes/cytology,physiology Lymphoma/pathology,physiopathology Mannosephosphates/metabolism Mice Receptor, IGF Type 2
Chemicals
Carrier Proteins Hexosephosphates Mannosephosphates Receptor, IGF Type 2 mannose-6-phosphate
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Yednock T A
Stoolman L M
Rosen S D
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42 references, click to expand
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.104.3.713
Published
1987-03-00
Pages
713-23
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2114559
Subset
IM
Grants
NCI NIH HHS · 1K08CA00959 · United States
NIGMS NIH HHS · GM23547 · United States
External Links
PubMed source DOI: 10.1083/jcb.104.3.713 PMC Full Text View journal details
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