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PMID: 2955416 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

B-cell variant of mouse T200 (Ly-5): evidence for alternative mRNA splicing.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 84 ·No. 15 ·1987-08-00 ·Pages 5360-3

Thomas ML, Reynolds PJ, Chain A, Ben-Neriah Y, Trowbridge IS

Abstract

The complete cDNA sequence of mouse T200 glycoprotein from the pre-B-cell line 70Z/3 has been determined. The deduced protein sequence differs from that previously reported for a T-cell form of the molecule [Saga, Y., Tung, J.-S., Shen, F.-W. & Boyse, E. A. (1986) Proc. Natl. Acad. Sci. USA 83, 6940-6944] by the insertion of 139 amino acid residues in the amino-terminal region of the molecule. RNA transfer blotting using a cDNA probe encoding this sequence established that the predominant T200 mRNA species from B cells and cytotoxic T-cell clones but not T-helper cell clones or thymocytes contain all or part of the insert. Overlapping genomic clones of murine T200 were isolated and analysis of the intron-exon structure at the 5' end of the gene provides evidence that variants of T200 glycoprotein are generated by alternative mRNA splicing.

MeSH Terms
Amino Acid Sequence Animals B-Lymphocytes/analysis Base Sequence DNA/analysis Histocompatibility Antigens/genetics Leukocyte Common Antigens Mice Molecular Weight RNA Splicing RNA, Messenger/metabolism
Chemicals
Histocompatibility Antigens RNA, Messenger DNA Leukocyte Common Antigens
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Thomas M L
Reynolds P J
Chain A
Ben-Neriah Y
Trowbridge I S
References (27)
27 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.84.15.5360
Published
1987-08-00
Pages
5360-3
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC298855
Subset
IM
Grants
NCI NIH HHS · CA 17733 · United States
Databases
GENBANK
J04640, J04691, M17216, M22455, M23126, M23127, M23128, M23129, M23130, M23131, M23132, M23133, M23134, M23135, M23136, M23137, M23138, M23139, M23140, M23141, M23142, M23143, M23144, M23145, M23146, M23147, M23148, M23149, M23150, M23151
External Links
PubMed source DOI: 10.1073/pnas.84.15.5360 PMC Full Text View journal details
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