Home LiteratureArticle Details
PMID: 2958481 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Amino acid sequence of the human fibronectin receptor.

The Journal of cell biology ·Vol. 105 ·No. 3 ·1987-09-00 ·Pages 1183-90

Argraves WS, Suzuki S, Arai H, Thompson K, Pierschbacher MD, Ruoslahti E

Abstract

The amino acid sequence deduced from cDNA of the human placental fibronectin receptor is reported. The receptor is composed of two subunits: an alpha subunit of 1,008 amino acids which is processed into two polypeptides disulfide bonded to one another, and a beta subunit of 778 amino acids. Each subunit has near its COOH terminus a hydrophobic segment. This and other sequence features suggest a structure for the receptor in which the hydrophobic segments serve as transmembrane domains anchoring each subunit to the membrane and dividing each into a large ectodomain and a short cytoplasmic domain. The alpha subunit ectodomain has five sequence elements homologous to consensus Ca2+-binding sites of several calcium-binding proteins, and the beta subunit contains a fourfold repeat strikingly rich in cysteine. The alpha subunit sequence is 46% homologous to the alpha subunit of the vitronectin receptor. The beta subunit is 44% homologous to the human platelet adhesion receptor subunit IIIa and 47% homologous to a leukocyte adhesion receptor beta subunit. The high degree of homology (85%) of the beta subunit with one of the polypeptides of a chicken adhesion receptor complex referred to as integrin complex strongly suggests that the latter polypeptide is the chicken homologue of the fibronectin receptor beta subunit. These receptor subunit homologies define a superfamily of adhesion receptors. The availability of the entire protein sequence for the fibronectin receptor will facilitate studies on the functions of these receptors.

MeSH Terms
Amino Acid Sequence Base Sequence DNA/isolation & purification Female Humans Macromolecular Substances Molecular Sequence Data Nucleic Acid Hybridization Placenta/immunology RNA, Messenger/genetics Receptors, Fibronectin Receptors, Immunologic/genetics
Chemicals
Macromolecular Substances RNA, Messenger Receptors, Fibronectin Receptors, Immunologic DNA
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Argraves W S
La Jolla Cancer Research Foundation, California 92037.
Suzuki S
Arai H
Thompson K
Pierschbacher M D
Ruoslahti E
References (48)
48 references, click to expand
  1. Detection of specific sequences among DNA fragments separated by gel electrophoresis.
    J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
  2. Protein sequence of endothelial glycoprotein IIIa derived from a cDNA clone. Identity with platelet glycoprotein IIIa and similarity to "integrin".
    J Biol Chem. 1987 Mar 25;262(9):3936-9 PMID: 3494014
  3. RNA molecular weight determinations by gel electrophoresis under denaturing conditions, a critical reexamination.
    Biochemistry. 1977 Oct 18;16(21):4743-51 PMID: 911786
  4. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  5. Analysis of the accuracy and implications of simple methods for predicting the secondary structure of globular proteins.
    J Mol Biol. 1978 Mar 25;120(1):97-120 PMID: 642007
  6. Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose.
    Proc Natl Acad Sci U S A. 1980 Sep;77(9):5201-5 PMID: 6159641
  7. Structure of vitamin D-dependent calcium-binding protein from bovine intestine.
    Nature. 1981 Nov 26;294(5839):327-32 PMID: 7312031
  8. Membrane glycoproteins involved in cell--substratum adhesion.
    Proc Natl Acad Sci U S A. 1981 Oct;78(10):6071-5 PMID: 6273845
  9. Mechanisms for the incorporation of proteins in membranes and organelles.
    J Cell Biol. 1982 Jan;92(1):1-22 PMID: 7035466
  10. Monoclonal antibodies which alter the morphology of cultured chick myogenic cells.
    J Cell Biochem. 1982;18(2):221-9 PMID: 7068780
  11. Purification of glycoproteins IIb and III from human platelet plasma membranes and characterization of a calcium-dependent glycoprotein IIb-III complex.
    J Biol Chem. 1982 Sep 10;257(17):10458-66 PMID: 6213621
  12. Efficient isolation of genes by using antibody probes.
    Proc Natl Acad Sci U S A. 1983 Mar;80(5):1194-8 PMID: 6219389
  13. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity.
    Anal Biochem. 1983 Jul 1;132(1):6-13 PMID: 6312838
  14. How signal sequences maintain cleavage specificity.
    J Mol Biol. 1984 Feb 25;173(2):243-51 PMID: 6423828
  15. Evidence that three adhesive proteins interact with a common recognition site on activated platelets.
    J Biol Chem. 1984 May 10;259(9):5388-91 PMID: 6325435
  16. Cell attachment activity of fibronectin can be duplicated by small synthetic fragments of the molecule.
    Nature. 1984 May 3-9;309(5963):30-3 PMID: 6325925
  17. Molecular cloning and sequence analysis of human placental alkaline phosphatase.
    J Biol Chem. 1986 Mar 5;261(7):3112-5 PMID: 3512548
  18. Rapid synthesis of cDNA for cloning into lambda vectors.
    Nucleic Acids Res. 1986 Feb 25;14(4):1913 PMID: 3005983
  19. Fibronectin receptor structures in the VLA family of heterodimers.
    Nature. 1987 Apr 9-15;326(6113):607-9 PMID: 3031508
  20. Immunochemical and amino-terminal sequence comparison of two cytoadhesins indicates they contain similar or identical beta subunits and distinct alpha subunits.
    J Biol Chem. 1987 Apr 25;262(12):5437-40 PMID: 2437107
  21. The very late antigen family of heterodimers is part of a superfamily of molecules involved in adhesion and embryogenesis.
    Proc Natl Acad Sci U S A. 1987 May;84(10):3239-43 PMID: 3033641
  22. The primary structure of the beta-subunit of the cell surface adhesion glycoproteins LFA-1, CR3 and p150,95 and its relationship to the fibronectin receptor.
    EMBO J. 1987 Apr;6(4):915-9 PMID: 2954816
  23. Calcium ions protect cell-substratum adhesion receptors against proteolysis. Evidence from immunoabsorption and electroblotting studies.
    Exp Cell Res. 1984 Jun;152(2):467-75 PMID: 6373329
  24. Compilation of published signal sequences.
    Nucleic Acids Res. 1984 Jul 11;12(13):5145-64 PMID: 6379599
  25. Cleavage of a 135 kD cell surface glycoprotein correlates with loss of fibroblast adhesion to fibronectin.
    Exp Cell Res. 1985 Jan;156(1):182-90 PMID: 2981173
  26. Inherited deficiency of the Mac-1, LFA-1, p150,95 glycoprotein family and its molecular basis.
    J Exp Med. 1984 Dec 1;160(6):1901-18 PMID: 6096477
  27. Identification and isolation of a 140 kd cell surface glycoprotein with properties expected of a fibronectin receptor.
    Cell. 1985 Jan;40(1):191-8 PMID: 3155652
  28. Selective inhibition of fibronectin-mediated cell adhesion by monoclonal antibodies to a cell-surface glycoprotein.
    Science. 1985 Jun 21;228(4706):1448-51 PMID: 4012302
  29. Synthetic peptides competitively inhibit both direct binding to fibroblasts and functional biological assays for the purified cell-binding domain of fibronectin.
    J Biol Chem. 1985 Sep 5;260(19):10402-5 PMID: 3161878
  30. A 125/115-kDa cell surface receptor specific for vitronectin interacts with the arginine-glycine-aspartic acid adhesion sequence derived from fibronectin.
    Proc Natl Acad Sci U S A. 1985 Sep;82(17):5766-70 PMID: 2412224
  31. The cell substrate attachment (CSAT) antigen has properties of a receptor for laminin and fibronectin.
    J Cell Biol. 1985 Dec;101(6):2134-44 PMID: 2933413
  32. p150,95, the third member of the Mac-1, LFA-1 human leukocyte adhesion glycoprotein family.
    J Immunol. 1986 Jan;136(1):240-5 PMID: 3510003
  33. The effect of Arg-Gly-Asp-containing peptides on fibrinogen and von Willebrand factor binding to platelets.
    Proc Natl Acad Sci U S A. 1985 Dec;82(23):8057-61 PMID: 3877935
  34. Characterization of toposomes from sea urchin blastula cells: a cell organelle mediating cell adhesion and expressing positional information.
    Proc Natl Acad Sci U S A. 1985 Dec;82(23):8062-6 PMID: 3865216
  35. Platelet membrane glycoprotein IIb/IIIa: member of a family of Arg-Gly-Asp--specific adhesion receptors.
    Science. 1986 Mar 28;231(4745):1559-62 PMID: 2420006
  36. Structure of integrin, a glycoprotein involved in the transmembrane linkage between fibronectin and actin.
    Cell. 1986 Jul 18;46(2):271-82 PMID: 3487386
  37. Site-directed mutagenesis of the regulatory light-chain Ca2+/Mg2+ binding site and its role in hybrid myosins.
    Nature. 1986 Jul 3-9;322(6074):80-3 PMID: 3523256
  38. cDNA sequences from the alpha subunit of the fibronectin receptor predict a transmembrane domain and a short cytoplasmic peptide.
    J Biol Chem. 1986 Oct 5;261(28):12922-4 PMID: 2944883
  39. Fibronectin-binding properties of the purified platelet glycoprotein IIb-IIIa complex.
    J Biol Chem. 1986 Oct 25;261(30):14011-7 PMID: 3771519
  40. cDNA and amino acid sequences of the cell adhesion protein receptor recognizing vitronectin reveal a transmembrane domain and homologies with other adhesion protein receptors.
    Proc Natl Acad Sci U S A. 1986 Nov;83(22):8614-8 PMID: 2430295
  41. The structure of human thrombospondin, an adhesive glycoprotein with multiple calcium-binding sites and homologies with several different proteins.
    J Cell Biol. 1986 Nov;103(5):1635-48 PMID: 2430973
  42. Integrin (the CSAT antigen): functionality requires oligomeric integrity.
    J Cell Biol. 1986 Dec;103(6 Pt 1):2421-8 PMID: 3491082
  43. Peptide inhibitors of fibronectin, laminin, and other adhesion molecules: unique and shared features.
    J Cell Physiol. 1987 Jan;130(1):21-8 PMID: 3805128
  44. Integrins: a family of cell surface receptors.
    Cell. 1987 Feb 27;48(4):549-54 PMID: 3028640
  45. Cloning of the beta subunit of the leukocyte adhesion proteins: homology to an extracellular matrix receptor defines a novel supergene family.
    Cell. 1987 Feb 27;48(4):681-90 PMID: 3028646
  46. Competition for related but nonidentical binding sites on the glycoprotein IIb-IIIa complex by peptides derived from platelet adhesive proteins.
    Cell. 1987 Mar 13;48(5):867-73 PMID: 3493077
  47. The VLA protein family. Characterization of five distinct cell surface heterodimers each with a common 130,000 molecular weight beta subunit.
    J Biol Chem. 1987 Mar 5;262(7):3300-9 PMID: 3546305
  48. Levels of translatable mRNAs for cell surface protein, collagen precursors, and two membrane proteins are altered in Rous sarcoma virus-transformed chick embryo fibroblasts.
    Proc Natl Acad Sci U S A. 1977 Aug;74(8):3399-403 PMID: 198783
Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.105.3.1183
Published
1987-09-00
Pages
1183-90
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2114793
Subset
IM
Grants
NCI NIH HHS · CA 28896 · United States
NCI NIH HHS · CA 30199 · United States
NCI NIH HHS · CA 42507 · United States
Databases
GENBANK
X06256, X07979
External Links
PubMed source DOI: 10.1083/jcb.105.3.1183 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2