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PMID: 29717166 Published · epublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Comparative Proteomics Reveal the Association between SPANX Proteins and Clinical Outcomes of Artificial Insemination with Donor Sperm.

Scientific reports ·Vol. 8 ·No. 1 ·2018-00-01 ·Pages 6850

Wang XM, Xiang Z, Fu Y, Wu HL, Zhu WB, Fan LQ

Abstract

Semen analysis is used for diagnosing male infertility and evaluating male fertility for more than a century. However, the semen analysis simply represents the population characteristics of sperm. It is not a comprehensive assessment of the male reproductive potential. In this study, 20 semen samples from human sperm bank with distinctive artificial insemination with donor sperm (AID) clinical outcomes were collected and analyzed using a two-dimensional differential in-gel electrophoresis (2D-DIGE); 45 differentially expressed protein spots were obtained, and 26 proteins were identified. Most differentially expressed proteins were related to sperm motility, energy consumption, and structure. These identified proteins included several sperm proteins associated with the nucleus on the X chromosome (SPANX) proteins. This prospective study aimed to investigate the association between the expression levels of SPANX proteins and the AID clinical outcomes. The proteins identified in this study provided a reference for the molecular mechanism of sperm fertility and revealed a predictive value of the SPANX proteins.

MeSH Terms
Adult Female Humans Infertility, Male/diagnosis Insemination, Artificial, Heterologous Male Nuclear Proteins/metabolism Prospective Studies Proteomics Semen/metabolism Semen Analysis/methods Sperm Motility Spermatozoa/metabolism Tissue Donors
Chemicals
Nuclear Proteins SPANXA1 protein, human
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Wang X M
Institute of Reproductive & Stem Cell Engineering, School of Basic Medicine Science, Central South University, Changsha, China.
Xiang Z
Shenzhen Armed Police Hospital Reproductive Center, Shenzhen, China.
Fu Y
Medical center for Human Reproduction, Beijing Chao-yang Hospital affiliated to Capital Medical University, Beijing, China.
Wu H L
Reproductive & Genetic Hospital of CITIC-Xiangya, Changsha, China.
Zhu W B
Institute of Reproductive & Stem Cell Engineering, School of Basic Medicine Science, Central South University, Changsha, China. | Reproductive & Genetic Hospital of CITIC-Xiangya, Changsha, China.
Fan L Q
Institute of Reproductive & Stem Cell Engineering, School of Basic Medicine Science, Central South University, Changsha, China. [email protected]. | Reproductive & Genetic Hospital of CITIC-Xiangya, Changsha, China. [email protected].
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Article Info
Journal
Scientific reports
Abbr.
Sci Rep
ISSN
2045-2322
DOI
10.1038/s41598-018-25032-4
Email
[email protected]
Published
2018-00-01
Epub
2018-00-01
Pages
6850
Language
English
Region
England
NLM ID
101563288
PMCID
PMC5931513
Subset
IM
External Links
PubMed source DOI: 10.1038/s41598-018-25032-4 PMC Full Text View journal details
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