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PMID: 2972910 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Increased expression of the Escherichia coli umuDC operon restores SOS mutagenesis in lexA41 cells.

Molecular & general genetics : MGG ·Vol. 213 ·No. 2-3 ·1988-08-00 ·Pages 541-4

Ennis DG, Peterson KR, Mount DW

Abstract

The lexA41 allele of Escherichia coli encodes a semidefective mutant repressor that is also resistant to RecA facilitated cleavage. Cells harboring the lexA41 allele were found previously to repress only a subset of operons in the SOS regulon. lexA41 cells cannot promote SOS mutagenesis, presumably because one or more operons required for mutagenesis are repressed by this mutant repressor. Using the lac regulatory system to increase the expression of the umuDC operon, we were able to restore mutagenesis in the lexA41 mutant. We conclude that the products of the umuDC operon appear to be uniquely limiting in this mutant.

MeSH Terms
Alleles Bacterial Proteins/genetics Bacteriophage lambda/genetics DNA Repair Escherichia coli/genetics,radiation effects Genes, Bacterial Mutation Operon SOS Response, Genetics Serine Endopeptidases Ultraviolet Rays
Chemicals
Bacterial Proteins LexA protein, Bacteria Serine Endopeptidases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Ennis D G
Department of Molecular and Cellular Biology, University of Arizona, Tucson 85721.
Peterson K R
Mount D W
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23 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00339628
Published
1988-08-00
Pages
541-4
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
Grants
NIGMS NIH HHS · GM24496 · United States
External Links
PubMed source DOI: 10.1007/BF00339628
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