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PMID: 2973060 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

T helper cell subsets require the expression of distinct costimulatory signals by antigen-presenting cells.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 85 ·No. 21 ·1988-11-00 ·Pages 8181-5

Weaver CT, Hawrylowicz CM, Unanue ER

Abstract

We examined the ability of macrophages and B cells to function as antigen-presenting cells (APCs) for murine TH1 and TH2 cloned T helper cell lines. Antigen presented by concanavalin A-elicited peritoneal macrophages or resting splenic B cells stimulated antigen-dependent proliferation of both T helper subsets. Paraformaldehyde fixation of the APCs following different conditions of activation indicated differential requirements for costimulatory signals by TH1 and TH2 cells. TH2 proliferative responses were strictly dependent on APC expression of IL-1. TH1 proliferation was dependent on APC expression of a non-IL-1 costimulatory signal present on freshly isolated macrophages and on splenic B cells activated with anti-immunoglobulin plus interferon gamma.

MeSH Terms
Animals Antigen-Presenting Cells/immunology Antigens, Differentiation, T-Lymphocyte/immunology B-Lymphocytes/immunology Cell Division Cell Line Concanavalin A/pharmacology Female Interferon-gamma/pharmacology Interleukin-1/pharmacology Macrophages/immunology Male Mice Mice, Inbred Strains T-Lymphocytes, Helper-Inducer/immunology
Chemicals
Antigens, Differentiation, T-Lymphocyte Interleukin-1 Concanavalin A Interferon-gamma
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Weaver C T
Department of Pathology, Washington University School of Medicine, Saint Louis, MO 63110.
Hawrylowicz C M
Unanue E R
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21 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.85.21.8181
Published
1988-11-00
Pages
8181-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC282391
Subset
IM
Grants
NIAID NIH HHS · AI22033 · United States
NIAID NIH HHS · AI24742 · United States
NIAID NIH HHS · AI24854 · United States
External Links
PubMed source DOI: 10.1073/pnas.85.21.8181 PMC Full Text View journal details
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