2-Aminopurine and 5-bromouracil induce alleviation of type I restriction in Escherichia coli: mismatches function as inducing signals?

Molecular & general genetics : MGG ·Vol. 214 ·No. 2 ·1988-10-00 ·Pages 317-20

Efimova EP, Delver EP, Belogurov AA

Abstract

The EcoK restriction of unmodified phage lambda is 1000-fold alleviated in Escherichia coli grown in the presence of base analogs 2-aminopurine (2AP) and 5-bromouracil (5BU). 2AP treatment of bacteria affects specifically the type I restriction systems (EcoA, EcoB, EcoD and EcoK) and does not influence type II (EcoRI) and type III (EcoP1) restriction. 2AP-induced alleviation of restriction occurs in bacteria which are deficient in the SOS response (recA and lexA) and mismatch repair (mutH, mutL and mutS) and can be distinguished from the alleviation of restriction observed in dam- strains. We suggest that mismatches induced by 2AP and 5BU may function as an inducing signal for the alleviation of restriction observed in the presence of base analogs.

MeSH Terms

2-Aminopurine/pharmacology Adenine/analogs & derivatives Bacteriophage lambda/genetics Bromouracil/pharmacology Deoxyribonucleases, Type I Site-Specific/genetics,metabolism Escherichia coli/drug effects,enzymology,genetics Genotype Phenotype

Chemicals

2-Aminopurine Bromouracil endodeoxyribonuclease EcoK Deoxyribonucleases, Type I Site-Specific Adenine

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Efimova E P

National Cardiology Research Center, USSR Academy of Medical Sciences, Moscow.

Delver E P

Belogurov A A

References (16)

16 references, click to expand

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Article Info

Journal

Molecular & general genetics : MGG

Abbr.

Mol Gen Genet

ISSN

0026-8925

DOI

10.1007/BF00337728

Published

1988-10-00

Pages

317-20

Language

English

Region

Germany

NLM ID

0125036

Subset

External Links

PubMed source DOI: 10.1007/BF00337728

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2-Aminopurine and 5-bromouracil induce alleviation of type I restriction in Escherichia coli: mismatches function as inducing signals?

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