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PMID: 2981230 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Tissue-specific expression of distinct spectrin and ankyrin transcripts in erythroid and nonerythroid cells.

The Journal of cell biology ·Vol. 100 ·No. 1 ·1985-01-00 ·Pages 152-60

Moon RT, Ngai J, Wold BJ, Lazarides E

Abstract

cDNA probes for three components of the erythroid membrane skeleton, alpha spectrin, beta spectrin, and ankyrin, were obtained by using monospecific antibodies to screen a lambda gt11 expression vector library containing cDNA prepared from chicken erythroid poly(A)+ RNA. Each cDNA appears to hybridize to one gene type in the chicken genome. Qualitatively distinct RNA species in myogenic and erythroid cells are detected for beta spectrin and ankyrin, while alpha spectrin exists as a single species of transcript in all tissues examined. This tissue-specific expression of RNAs is regulated quantitatively during myogenesis in vitro, since all three accumulate only upon myoblast fusion. Furthermore, RNAs for two of the three genes do not accumulate to detectable levels in chicken embryo fibroblasts, demonstrating that their accumulation can be noncoordinate. These observations suggest that independent gene regulation and tissue-specific production of heterogeneous transcripts from the beta spectrin and ankyrin genes underlie the formation of distinct membrane skeletons in erythroid and muscle cells.

MeSH Terms
Animals Ankyrins Cells, Cultured Chick Embryo Cloning, Molecular DNA/metabolism DNA Restriction Enzymes Genes Membrane Proteins/genetics Muscles/cytology,metabolism Nucleic Acid Hybridization Protein Biosynthesis Spectrin/genetics Transcription, Genetic
Chemicals
Ankyrins Membrane Proteins Spectrin DNA DNA Restriction Enzymes
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Moon R T
Ngai J
Wold B J
Lazarides E
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52 references, click to expand
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.100.1.152
Published
1985-01-00
Pages
152-60
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2113487
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.100.1.152 PMC Full Text View journal details
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