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PMID: 2981822 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Immunological investigation of the distribution of cytochromes related to the two terminal oxidases of Escherichia coli in other gram-negative bacteria.

Journal of bacteriology ·Vol. 161 ·No. 2 ·1985-02-00 ·Pages 709-13

Kranz RG, Gennis RB

Abstract

Monospecific antibodies were raised against the two terminal oxidase complexes of the aerobic respiratory chain of Escherichia coli. These are the cytochrome d and cytochrome o complexes. The antibodies were used to check for the occurrence of cross-reactive antigens in membrane preparations from a variety of gram-negative bacteria by rocket immunoelectrophoresis and immunoblotting techniques. With these criteria, proteins closely related to the cytochrome d complex of E. coli appeared to be widely distributed. Among the strains containing cytochrome d-related material were Serratia marcescens, Photobacterium phosphoreum, Salmonella typhimurium, Klebsiella pneumoniae, and Azotobacter vinelandii. The data suggest that the d-type terminal oxidase in many of these strains is associated in a complex with b-type and a1-type cytochromes, as has been found to be the case in E. coli. K. pneumoniae and S. typhimurium were also shown to have material cross-reactive to the E. coli cytochrome o complex.

MeSH Terms
Cross Reactions Cytochrome b Group Cytochrome d Group Cytochromes/analysis,immunology Escherichia coli/enzymology Escherichia coli Proteins Gram-Negative Bacteria/enzymology Immunoelectrophoresis Oxidoreductases/analysis
Chemicals
Cytochrome b Group Cytochromes Escherichia coli Proteins Cytochrome d Group cytochrome bo, E coli Oxidoreductases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Kranz R G
Gennis R B
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51 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.161.2.709-713.1985
Published
1985-02-00
Pages
709-13
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC214940
Subset
IM
Grants
NHLBI NIH HHS · HL16101 · United States
External Links
PubMed source DOI: 10.1128/jb.161.2.709-713.1985 PMC Full Text View journal details
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