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PMID: 2982788 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Analysis of mobilization elements in plasmids from Shigella flexneri.

Journal of bacteriology ·Vol. 161 ·No. 3 ·1985-03-00 ·Pages 1233-5

Roessler E, Fenwick RG, Chinault AC

Abstract

The mobilization properties of three plasmids were examined after cotransfer from Shigella flexneri to Escherichia coli. The largest plasmid, pCN1, was shown to be a conjugative R factor that could promote its own transfer and allow cotransfer of a 4.1-kilobase plasmid, pCN3; mobilization of the third plasmid, pCN2 (6.3 kilobases), required the presence of both pCN1 and pCN3. Sequences from pCN2 and pCN3 homologous to the bom (basis of mobilization) sites of ColE1 and pBR322 were localized by analysis of site-specific deletion derivatives generated in vivo during the transfer of composite plasmids and were characterized by DNA sequencing.

MeSH Terms
Base Sequence DNA Restriction Enzymes Drug Resistance, Microbial Escherichia coli/genetics Plasmids R Factors Recombination, Genetic Shigella flexneri/genetics
Chemicals
DNA Restriction Enzymes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Roessler E
Fenwick R G
Chinault A C
References (12)
12 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.161.3.1233-1235.1985
Published
1985-03-00
Pages
1233-5
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC215035
Subset
IM
Databases
GENBANK
M10183, M10184
External Links
PubMed source DOI: 10.1128/jb.161.3.1233-1235.1985 PMC Full Text View journal details
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