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PMID: 2982790 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Purification and characterization of the flagellar hook-basal body complex of Salmonella typhimurium.

Journal of bacteriology ·Vol. 161 ·No. 3 ·1985-03-00 ·Pages 836-49

Aizawa SI, Dean GE, Jones CJ, Macnab RM, Yamaguchi S

Abstract

The hook-basal body complex of Salmonella typhimurium, a major component of its flagellar apparatus, was subjected to detailed analysis by electron microscopy and gel electrophoresis. The study was facilitated by the development of an improved protocol for isolation of the complexes in high yield and purity. Nine proteins were identified with the structure. These proteins had apparent molecular weights of 65,000 (65K), 60K, 42K, 38K, 32K, 30K, 27K, 16K, and 14K. Small but reproducible shifts in the apparent molecular weights of specific proteins from conditionally nonflagellate mutants indicated the following gene-polypeptide correspondences: flaFV, 42K; flaFVI, 32K; flaFVII, 30K; flaFIX, 38K; flaAII.1, 65K. Several new morphological features of hook-basal body complexes were recognized, including a clawlike structure on the cytoplasm-proximal M ring and additional material at the cytoplasmic face of the M ring. Based on this study and the work of others, we suggest that the morphological features of the hook-basal body complex correspond to the following proteins: hook-filament junction, 60K; hook, 42K; rod, 30K and 32K; L ring and outer cylinder wall, 27K; P ring, 38K; S ring, unknown; M ring 65K.

MeSH Terms
Bacterial Outer Membrane Proteins/isolation & purification Bacterial Proteins/genetics,isolation & purification Cell Fractionation/methods Flagellin/isolation & purification Microscopy, Electron Molecular Weight Porins Salmonella typhimurium/genetics,ultrastructure
Chemicals
Bacterial Outer Membrane Proteins Bacterial Proteins Porins Flagellin
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Aizawa S I
Dean G E
Jones C J
Macnab R M
Yamaguchi S
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57 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.161.3.836-849.1985
Published
1985-03-00
Pages
836-49
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC214974
Subset
IM
Grants
NIAID NIH HHS · AI12202 · United States
External Links
PubMed source DOI: 10.1128/jb.161.3.836-849.1985 PMC Full Text View journal details
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