Archaebacterial malate dehydrogenases. The enzymes from the thermoacidophilic organisms Sulfolobus acidocaldarius and Thermoplasma acidophilum show A-side stereospecificity for NAD+.

The Biochemical journal ·Vol. 226 ·No. 3 ·1985-03-15 ·Pages 885-8

Görisch H, Hartl T, Grossebüter W, Stezowski JJ

Abstract

The stereoselective transfer of hydrogen from NADH to oxaloacetate catalysed by malate dehydrogenases (EC 1.1.1.37) from the thermoacidophilic archaebacteria Sulfolobus acidocaldarius and Thermoplasma acidophilum was studied by the p.m.r. method described by Zhou & Wong [(1981) J. Biochem. Biophys. Methods 4, 329-338]. Both enzymes are A-side (pro-R) stereospecific for NADH.

MeSH Terms

Archaea/enzymology Bacteria/enzymology Binding Sites Electron Spin Resonance Spectroscopy Malate Dehydrogenase/metabolism Molecular Conformation NAD/metabolism

Chemicals

NAD Malate Dehydrogenase

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Görisch H

Hartl T

Grossebüter W

Stezowski J J

References (6)

6 references, click to expand

Studies on the biosynthesis of cholesterol. XVII. The asymmetric synthesis of a symmetrical molecule.
Proc R Soc Lond B Biol Sci. 1966 Jan 18;163(993):437-64 PMID: 4379289
Sulfolobus: a new genus of sulfur-oxidizing bacteria living at low pH and high temperature.
Arch Mikrobiol. 1972;84(1):54-68 PMID: 4559703
Phylogenetic structure of the prokaryotic domain: the primary kingdoms.
Proc Natl Acad Sci U S A. 1977 Nov;74(11):5088-90 PMID: 270744
NMR determination of hydrogen transfer stereospecificity of alpha-keto acid dehydrogenase complexes: a one-step method.
J Biochem Biophys Methods. 1981 Jun;4(5-6):329-38 PMID: 7019297
The stereoselectivity of alcohol dehydrogenases: a stereochemical imperative?
Experientia. 1982 May 15;38(5):633-6 PMID: 7047206
Stereospecificities of the pyridine nucleotide-linked enzymes.
Methods Enzymol. 1982;87:101-26 PMID: 6757650

Article Info

Journal

The Biochemical journal

Abbr.

Biochem J

ISSN

0264-6021

DOI

10.1042/bj2260885

Published

1985-03-15

Pages

885-8

Language

English

Region

England

NLM ID

2984726R

PMCID

PMC1144792

Subset

External Links

PubMed source DOI: 10.1042/bj2260885 PMC Full Text View journal details

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Archaebacterial malate dehydrogenases. The enzymes from the thermoacidophilic organisms Sulfolobus acidocaldarius and Thermoplasma acidophilum show A-side stereospecificity for NAD+.

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