1,2-Diacylglycerol and phorbol ester inhibit agonist-induced formation of inositol phosphates in human platelets: possible implications for negative feedback regulation of inositol phospholipid hydrolysis.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 82 ·No. 9 ·1985-05-00 ·Pages 2623-6

Watson SP, Lapetina EG

Abstract

The present study has demonstrated that pretreatment of human platelets with either phorbol ester or 1,2-diacylglycerol inhibits agonist-induced formation of inositol phosphates; this inhibition can be correlated with a decrease in the release of ATP and 5-hydroxytryptamine by thrombin. The mechanism of this action is not known, but a role for protein kinase C is suggested, as both phorbol ester and 1,2-diacylglycerol have in common the ability to activate this enzyme. These results have important implications as a possible negative feedback control over agonist-induced hydrolysis of inositol phospholipids.

MeSH Terms

Blood Platelets/drug effects,metabolism Diglycerides/pharmacology Feedback Glycerides/pharmacology Humans Hydrolysis In Vitro Techniques Inositol Phosphates/blood Kinetics Phorbol 12,13-Dibutyrate Phorbol Esters/pharmacology Phorbols/pharmacology Phosphatidylinositol 4,5-Diphosphate Phosphatidylinositol Phosphates Phosphatidylinositols/blood Sugar Phosphates/blood Thrombin/pharmacology

Chemicals

Diglycerides Glycerides Inositol Phosphates Phorbol Esters Phorbols Phosphatidylinositol 4,5-Diphosphate Phosphatidylinositol Phosphates Phosphatidylinositols Sugar Phosphates phosphatidylinositol 4-phosphate Phorbol 12,13-Dibutyrate 1-oleoyl-2-acetylglycerol Thrombin

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Watson S P

Lapetina E G

References (21)

21 references, click to expand

The rapid formation of inositol phosphates in human platelets by thrombin is inhibited by prostacyclin.
J Biol Chem. 1984 Nov 10;259(21):13199-203 PMID: 6386811
Phorbol myristate acetate stimulates formation of phosphatidyl inositol 4-phosphate and phosphatidyl inositol 4,5-bisphosphate in human platelets.
Biochem Biophys Res Commun. 1984 Oct 30;124(2):507-13 PMID: 6093804
myo-Inositol 1,4,5-trisphosphate stimulates protein phosphorylation in saponin-permeabilized human platelets.
Proc Natl Acad Sci U S A. 1984 Dec;81(23):7431-5 PMID: 6438636
Release of 14C-serotonin during initial platelet changes induced by thrombin, collagen, or A23187.
Blood. 1977 Nov;50(5):915-26 PMID: 332258
Rapid decrease of phosphatidylinositol 4,5-bisphosphate in thrombin-stimulated platelets.
J Biol Chem. 1982 Nov 10;257(21):12705-8 PMID: 6290478
Thrombin-induced phosphodiesteratic cleavage of phosphatidylinositol bisphosphate in human platelets.
J Biol Chem. 1983 Feb 25;258(4):2076-8 PMID: 6296123
Platelet-activating factor stimulates metabolism of phosphoinositides in horse platelets: possible relationship to Ca2+ mobilization during stimulation.
Proc Natl Acad Sci U S A. 1983 Feb;80(4):965-8 PMID: 6341992
The role of phospholipase C in platelet responses.
Life Sci. 1983 Sep 12;33(11):1011-8 PMID: 6412009
Diacylglycerol and phorbol ester stimulate secretion without raising cytoplasmic free calcium in human platelets.
Nature. 1983 Sep 22-28;305(5932):317-9 PMID: 6621685
Release of Ca2+ from a nonmitochondrial intracellular store in pancreatic acinar cells by inositol-1,4,5-trisphosphate.
Nature. 1983 Nov 3-9;306(5938):67-9 PMID: 6605482
myo-Inositol 1,4,5-trisphosphate. A second messenger for the hormonal mobilization of intracellular Ca2+ in liver.
J Biol Chem. 1984 Mar 10;259(5):3077-81 PMID: 6607924
Phosphatidylinositol 4,5-bisphosphate may represent the site of release of plasma membrane-bound calcium upon stimulation of human platelets.
Biochem Biophys Res Commun. 1984 Apr 16;120(1):226-31 PMID: 6324792
The role of protein kinase C in cell surface signal transduction and tumour promotion.
Nature. 1984 Apr 19-25;308(5961):693-8 PMID: 6232463
The second messenger linking receptor activation to internal Ca release in liver.
Nature. 1984 May 3-9;309(5963):63-6 PMID: 6325926
Rapid mobilization of Ca2+ from rat insulinoma microsomes by inositol-1,4,5-trisphosphate.
Nature. 1984 Jun 7-13;309(5968):562-4 PMID: 6328320
1,2-Diacylglycerols do not potentiate the action of phospholipases A2 and C in human platelets.
Biochem Biophys Res Commun. 1984 May 31;121(1):386-91 PMID: 6732815
12-O-Tetradecanoylphorbol 13-acetate stimulates inositol lipid phosphorylation in intact human platelets.
FEBS Lett. 1984 Aug 6;173(2):389-93 PMID: 6235130
Diacylglycerols enhance human neutrophil degranulation responses: relevancy to a multiple mediator hypothesis of cell function.
Biochem Biophys Res Commun. 1984 Aug 30;123(1):64-70 PMID: 6433920
Leukotriene B4 mobilizes calcium without the breakdown of polyphosphoinositides and the production of phosphatidic acid in rabbit neutrophils.
Proc Natl Acad Sci U S A. 1984 Oct;81(19):5966-9 PMID: 6091126
1-Oleoyl-2-acetyl-glycerol (OAG) stimulates the formation of phosphatidylinositol 4-phosphate in intact human platelets.
Biochem Biophys Res Commun. 1984 Sep 17;123(2):589-95 PMID: 6091634
Phorbol esters inhibit agonist-induced [3H] inositol-1-phosphate accumulation in rat hippocampal slices.
Biochem Biophys Res Commun. 1984 Sep 17;123(2):703-9 PMID: 6593069

Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.82.9.2623

Published

1985-05-00

Pages

2623-6

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC397616

Subset

External Links

PubMed source DOI: 10.1073/pnas.82.9.2623 PMC Full Text View journal details

Analysis Services

1,2-Diacylglycerol and phorbol ester inhibit agonist-induced formation of inositol phosphates in human platelets: possible implications for negative feedback regulation of inositol phospholipid hydrolysis.

Contact

WeChat Official Account