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PMID: 2986186 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Nucleotide sequence analysis of a gene encoding a streptomycin/spectinomycin adenylyltransferase.

Plasmid ·Vol. 13 ·No. 1 ·1985-01-00 ·Pages 17-30

Hollingshead S, Vapnek D

Abstract

The nucleotide sequence of 1400 bp from R-plasmid R538-1 containing the streptomycin/spectinomycin adenyltransferase gene (aadA) was determined, and the location of the aadA gene was identified by a combination of insertion and deletion mutants. Its gene product, aminoglycoside 3"-adenylyltransferase (AAD(3")(9), has a Mr of 31,600.

MeSH Terms
Amino Acid Sequence Bacterial Proteins/genetics Base Sequence Codon/genetics DNA, Bacterial/genetics Escherichia coli/enzymology,genetics Genes Genes, Bacterial Nucleotidyltransferases/genetics R Factors
Chemicals
Bacterial Proteins Codon DNA, Bacterial Nucleotidyltransferases streptomycin-spectinomycin adenylyltransferase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Hollingshead S
Vapnek D
Article Info
Journal
Plasmid
Abbr.
Plasmid
ISSN
0147-619X
DOI
10.1016/0147-619x(85)90052-6
Published
1985-01-00
Pages
17-30
Language
English
Region
United States
NLM ID
7802221
Subset
IM
Databases
GENBANK
M10241
External Links
PubMed source DOI: 10.1016/0147-619x(85)90052-6 View journal details
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