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PMID: 2987646 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Homologous recombination of polyoma virus DNA in mouse cells.

Molecular & general genetics : MGG ·Vol. 199 ·No. 1 ·1985-00-00 ·Pages 146-51

Kovar H, Wintersberger E

Abstract

We have produced nonviable deletion mutants of polyoma virus in order to study homologous recombination after DNA transfection into mouse cells. The frequency of recombination was determined by the formation of infectious virus. It was dependent on the amount of DNA transfected and the size of the region of homology between the mutations. Recombination frequencies were highest when both mutated genomes were transfected in closed circular form rather than after linearization of one or both of the recombination partners. The system described may be useful for a more detailed analysis of physiological and genetic conditions influencing the frequency of homologous recombination in mouse cells as well as to study enzymes involved and intermediates produced in this process.

MeSH Terms
Animals Cells, Cultured Chromosome Deletion DNA, Circular/genetics DNA, Viral/genetics Defective Viruses/genetics Mice Polyomavirus/genetics Recombination, Genetic Transfection Virus Replication
Chemicals
DNA, Circular DNA, Viral
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Kovar H
Wintersberger E
References (37)
37 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00327524
Published
1985-00-00
Pages
146-51
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00327524
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