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PMID: 2987841 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Cloning and nucleotide sequence of the aspartase gene of Escherichia coli W.

Nucleic acids research ·Vol. 13 ·No. 6 ·1985-03-25 ·Pages 2063-74

Takagi JS, Ida N, Tokushige M, Sakamoto H, Shimura Y

Abstract

The aspA gene of Escherichia coli W which encodes aspartase was cloned into the plasmid vector pBR322. The nucleotide sequences of aspA and its flanking regions were determined. The aspA gene encodes a protein with a molecular weight of 52,224 consisted of 477 amino acid residues. The amino acid sequence of the protein predicted from the nucleotide sequence was consistent with those of the NH2- and COOH-terminal regions and also with the amino acid composition of the purified aspartase determined previously. Potential promoter and terminator sequences for aspA were also found in the determined sequence.

MeSH Terms
Amino Acid Sequence Ammonia-Lyases/genetics Aspartate Ammonia-Lyase/genetics Base Sequence Cloning, Molecular DNA Restriction Enzymes DNA, Bacterial/genetics Escherichia coli/genetics Genes, Bacterial Genes, Regulator Genetic Linkage Promoter Regions, Genetic
Chemicals
DNA, Bacterial DNA Restriction Enzymes Ammonia-Lyases Aspartate Ammonia-Lyase
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Takagi J S
Ida N
Tokushige M
Sakamoto H
Shimura Y
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21 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/13.6.2063
Published
1985-03-25
Pages
2063-74
Language
English
Region
England
NLM ID
0411011
PMCID
PMC341135
Subset
IM
Databases
GENBANK
X02307
External Links
PubMed source DOI: 10.1093/nar/13.6.2063 PMC Full Text View journal details
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