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PMID: 2987924 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Generation of a dominant 8-MDa deletion at the left terminus of vaccinia virus DNA.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 82 ·No. 10 ·1985-05-00 ·Pages 3365-9

Paez E, Dallo S, Esteban M

Abstract

Vaccinia virus mutants were obtained in high frequency from mouse Friend erythroleukemia (FEL) cells persistently infected with this virus, which contains a large (122-MDa) DNA. During long-term cell passages viral particles with deletions of the DNA are generated in FEL cells. These mutants have a major 8-MDa deletion starting between 2.2 and 3.2 MDa from the left terminus of the viral genome. More than half of the left end terminal repetition is deleted. These mutants have reduced infectivity compared to wild-type virus. The ease with which vaccinia virus mutants are obtained in FEL cells should provide a suitable system for generating mutants with other poxvirus and permit study of the genetic basis of virulence for this group of viruses.

MeSH Terms

Animals Cells, Cultured Chromosome Deletion DNA Restriction Enzymes DNA, Viral/genetics Leukemia, Erythroblastic, Acute/microbiology Mutation Vaccinia virus/genetics,pathogenicity Virus Replication

Chemicals

DNA, Viral DNA Restriction Enzymes

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Paez E

Dallo S

Esteban M

References (27)

27 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.82.10.3365

Published

1985-05-00

Pages

3365-9

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC397776

Subset

Grants

NIAID NIH HHS · R02 AI 16780 · United States

External Links

PubMed source DOI: 10.1073/pnas.82.10.3365 PMC Full Text View journal details

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Generation of a dominant 8-MDa deletion at the left terminus of vaccinia virus DNA.

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