Home LiteratureArticle Details

PMID: 2987994 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Plasmids related to the broad host range vector, pRK290, useful for gene cloning and for monitoring gene expression.

Plasmid ·Vol. 13 ·No. 2 ·1985-03-00 ·Pages 149-53

Ditta G, Schmidhauser T, Yakobson E, Lu P, Liang XW, Finlay DR, Guiney D, Helinski DR

Abstract

Derivatives of plasmid pRK290 that are useful for cloning and for analyzing gene expression in a wide variety of Gram-negative bacteria are described. A smaller broad host range plasmid derived from RK2, with properties similar to that of pRK290, is also described.

MeSH Terms

Cloning, Molecular DNA Restriction Enzymes Escherichia coli/enzymology,genetics Gene Expression Regulation Genes, Bacterial Genetic Vectors Lac Operon Plasmids beta-Galactosidase/genetics

Chemicals

DNA Restriction Enzymes beta-Galactosidase

Authors & Affiliations

8 authors, click to expand affiliations / ORCID

Ditta G

Schmidhauser T

Yakobson E

Lu P

Liang X W

Finlay D R

Guiney D

Helinski D R

Article Info

Journal

Plasmid

Abbr.

Plasmid

ISSN

0147-619X

DOI

10.1016/0147-619x(85)90068-x

Published

1985-03-00

Pages

149-53

Language

English

Region

United States

NLM ID

7802221

Subset

Grants

NIGMS NIH HHS · GM 28924 · United States

External Links

PubMed source DOI: 10.1016/0147-619x(85)90068-x View journal details

Analysis Services

Plasmids related to the broad host range vector, pRK290, useful for gene cloning and for monitoring gene expression.

Contact

WeChat Official Account