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PMID: 2989551 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Nucleotide sequence and structural features of a novel US-a junction present in a defective herpes simplex virus genome.

Journal of virology ·Vol. 55 ·No. 1 ·1985-07-00 ·Pages 140-6

Mocarski ES, Deiss LP, Frenkel N

Abstract

Defective genomes generated during serial propagation of herpes simplex virus type 1 (Justin) consist of tandem reiterations of sequences that are colinear with a portion of the S component of the standard viral genome. We determined the structure of the novel US-a junction, at which the US sequences of one repeat unit join the a sequences of the adjacent repeat unit. Comparison of the nucleotide sequence at this junction with the nucleotide sequence of the corresponding US region of the standard virus genome indicated that the defective genome repeat unit arose by a single recombinational event between an L-S junction a sequence and the US region. The recombinational process might have been mediated by limited sequence homology. The sequences retained within the US-a junction further define the signal for cleavage and packaging of viral DNA.

MeSH Terms
Base Sequence Cloning, Molecular DNA, Viral/genetics Defective Viruses/genetics Genes, Viral Morphogenesis Nucleic Acid Conformation Nucleic Acid Precursors/genetics Simplexvirus/genetics Virus Replication
Chemicals
DNA, Viral Nucleic Acid Precursors
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Mocarski E S
Deiss L P
Frenkel N
References (31)
31 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.55.1.140-146.1985
Published
1985-07-00
Pages
140-6
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC254908
Subset
IM
Grants
NIAID NIH HHS · AI-15488 · United States
NIAID NIH HHS · AI-20211 · United States
Databases
GENBANK
M10963
External Links
PubMed source DOI: 10.1128/JVI.55.1.140-146.1985 PMC Full Text View journal details
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