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PMID: 2989791 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Physical and functional structure of a yeast plasmid, pSB3, isolated from Zygosaccharomyces bisporus.

Nucleic acids research ·Vol. 13 ·No. 12 ·1985-06-25 ·Pages 4267-83

Toh-e A, Utatsu I

Abstract

The plasmid pSB3 of yeast Zygosacharomyces bisporus has been sequenced. It contains 6,615 base pairs, including a pair of inverted repeats (IR) consisting of 391 base pairs and 3 large open reading frames (ORF). One of the ORFs (A gene) participates in the recombination at the IRs and the other two (B and C genes) are necessary for the stable maintenance of this plasmid. The ARS sequence, which functions in a Saccharomyces cerevisiae host, was localized within 168 base pairs consisting of part of one of the IRs and a unique sequence contiguous to it. pSB3 can be maintained as stably in Z. rouxii as in the natural host Z.bisporus. In contrast, pSB3 is maintained fairly unstably in S.cerevisiae. The reason for this instability was found to be inefficient partitioning of pSB3 in S.cerevisiae. The molecular construction of pSB3 resembles that of 2-micron DNA, however, sequence homology at the DNA level was very poor.

MeSH Terms

Base Composition Base Sequence DNA Restriction Enzymes Genes, Fungal Molecular Sequence Data Nucleic Acid Conformation Plasmids Saccharomyces cerevisiae/genetics Saccharomycetales/genetics Species Specificity

Chemicals

DNA Restriction Enzymes

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Toh-e A

Utatsu I

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26 references, click to expand

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Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/13.12.4267

Published

1985-06-25

Pages

4267-83

Language

English

Region

England

NLM ID

0411011

PMCID

PMC321787

Subset

Databases

GENBANK

X02608

External Links

PubMed source DOI: 10.1093/nar/13.12.4267 PMC Full Text View journal details

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Physical and functional structure of a yeast plasmid, pSB3, isolated from Zygosaccharomyces bisporus.

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