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PMID: 2990894 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Amber, ochre and opal suppressor tRNA genes derived from a human serine tRNA gene.

The EMBO journal ·Vol. 4 ·No. 1 ·1985-01-00 ·Pages 213-21

Capone JP, Sharp PA, RajBhandary UL

Abstract

Amber, ochre and opal suppressor tRNA genes have been generated by using oligonucleotide directed site-specific mutagenesis to change one or two nucleotides in a human serine tRNA gene. The amber and ochre suppressor (Su+) tRNA genes are efficiently expressed in CV-1 cells when introduced as part of a SV40 recombinant. The expressed amber and ochre Su+ tRNAs are functional as suppressors as demonstrated by readthrough of the amber codon which terminates the NS1 gene of an influenza virus or the ochre codon which terminates the hexon gene of adenovirus, respectively. Interestingly, several attempts to obtain the equivalent virus stock of an SV40 recombinant containing the opal suppressor tRNA gene yielded virus lacking the opal suppressor tRNA gene. This suggests that expression of an efficient opal suppressor derived from a human serine tRNA gene is highly detrimental to either cellular or viral processes.

MeSH Terms
Animals Base Sequence Cloning, Molecular Codon/genetics Gene Expression Regulation Humans Mutation RNA, Transfer, Amino Acyl/genetics Simian virus 40/genetics Suppression, Genetic
Chemicals
Codon RNA, Transfer, Amino Acyl
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Capone J P
Sharp P A
RajBhandary U L
References (30)
30 references, click to expand
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1985-01-00
Pages
213-21
Language
English
Region
England
NLM ID
8208664
PMCID
PMC554172
Subset
IM
Grants
NCI NIH HHS · CA14051 · United States
NIGMS NIH HHS · GM17151 · United States
NIGMS NIH HHS · GM32467 · United States
External Links
PubMed source PMC Full Text View journal details
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