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PMID: 2991155 Published · ppublish English Case Reports Journal Article Research Support, Non-U.S. Gov't

Analysis of HSV isolated from patients with unilateral and bilateral herpetic keratitis.

International ophthalmology ·Vol. 8 ·No. 1 ·1985-04-00 ·Pages 13-8

Tognon M, Manservigi R, Sebastiani A, Bragliani G, Busin M, Cassai E

Abstract

Restriction enzyme analysis was utilized to investigate 11 HSV isolates from patients with herpetic keratitis. The electrophoretic profiles of the DNAs indicate that the isolates belong to HSV-1 and are epidemiologically unrelated. Two of the isolates, derived from a patient with simultaneous bilateral herpetic keratitis were further characterized by genetic and phenotypic analyses. These studies showed the following: (i) the two recrudescent lesions were caused by the same HSV-1 isolate; (ii) the isolate yielded two different plaque phenotypes; (iii) these plaques showed different sensitivity to I.D.U.

MeSH Terms

Acyclovir/therapeutic use Adult DNA Restriction Enzymes DNA, Viral/analysis Drug Resistance, Microbial Electrophoresis Herpes Simplex Humans Idoxuridine/therapeutic use Keratitis/drug therapy,etiology,microbiology Male Simplexvirus/analysis,classification,isolation & purification

Chemicals

DNA, Viral DNA Restriction Enzymes Idoxuridine Acyclovir

Authors & Affiliations

6 authors, click to expand affiliations / ORCID

Tognon M

Manservigi R

Sebastiani A

Bragliani G

Busin M

Cassai E

References (10)

10 references, click to expand

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Preparation of herpes simplex virus of high titer.
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J Virol. 1981 Jan;37(1):191-206 PMID: 6260973
Plaque dissociation of herpes simplex viruses: biochemical and biological characters of the viral variants.
Intervirology. 1975-1976;6(4-5):212-23 PMID: 186433
A new method for the isolation of herpes simplex virus type 2 DNA.
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9-(2-hydroxyethoxymethyl) guanine activity against viruses of the herpes group.
Nature. 1978 Apr 13;272(5654):583-5 PMID: 205792
BamI, KpnI, and SalI restriction enzyme maps of the DNAs of herpes simplex virus strains Justin and F: occurrence of heterogeneities in defined regions of the viral DNA.
J Virol. 1979 Nov;32(2):429-41 PMID: 228068
Randomised double-blind trial of acyclovir and idoxuridine in dendritic corneal ulceration.
Br J Ophthalmol. 1980 Oct;64(10):766-9 PMID: 7000171

Article Info

Journal

International ophthalmology

Abbr.

Int Ophthalmol

ISSN

0165-5701

DOI

10.1007/BF00136456

Published

1985-04-00

Pages

13-8

Language

English

Region

Netherlands

NLM ID

7904294

Subset

External Links

PubMed source DOI: 10.1007/BF00136456 View journal details

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Analysis of HSV isolated from patients with unilateral and bilateral herpetic keratitis.

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